Micronuclei in cytokinesis-blocked lymphocytes of cancer patients following fractionated partial-body radiotherapy

M. Fenech; J. Denham; W. Francis; A. Morley

doi:10.1080/09553009014552471

Micronuclei in cytokinesis-blocked lymphocytes of cancer patients following fractionated partial-body radiotherapy

M. Fenech, J. Denham, W. Francis, A. Morley

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Abstract

We applied the cytokinesis-block micronucleus assay to measure chromosome damage in lymphocytes of 11 cancer patients undergoing fractionated partial-body irradiation. Measurements performed before, during and after cessation of radiotherapy showed a dose-related increase in micronucleus frequency in each of the patients studied. When the results for micronucleus frequency (Y) were plotted against the estimated equivalent whole-body dose (X) the dose-response relationship obtained was Y = 75·8X + 49·5 (r = 0·783, P < 0·0001). A general decline in MN frequency was observed during the post-treatment period down to 57 per cent (± 10) after 12 months but there was considerable variation between individuals. The advantages and disadvantages of the application of the cytokinesis-block micronucleus assay as a biological dosemeter for lymphocytes irradiated in vivo are discussed.

Original language	English
Pages (from-to)	373-383
Number of pages	11
Journal	International Journal of Radiation Biology
Volume	57
Issue number	2
DOIs	https://doi.org/10.1080/09553009014552471
Publication status	Published - 1990
Externally published	Yes

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abstract = "We applied the cytokinesis-block micronucleus assay to measure chromosome damage in lymphocytes of 11 cancer patients undergoing fractionated partial-body irradiation. Measurements performed before, during and after cessation of radiotherapy showed a dose-related increase in micronucleus frequency in each of the patients studied. When the results for micronucleus frequency (Y) were plotted against the estimated equivalent whole-body dose (X) the dose-response relationship obtained was Y = 75·8X + 49·5 (r = 0·783, P < 0·0001). A general decline in MN frequency was observed during the post-treatment period down to 57 per cent (± 10) after 12 months but there was considerable variation between individuals. The advantages and disadvantages of the application of the cytokinesis-block micronucleus assay as a biological dosemeter for lymphocytes irradiated in vivo are discussed.",

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AU - Francis, W.

AU - Morley, A.

PY - 1990

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AB - We applied the cytokinesis-block micronucleus assay to measure chromosome damage in lymphocytes of 11 cancer patients undergoing fractionated partial-body irradiation. Measurements performed before, during and after cessation of radiotherapy showed a dose-related increase in micronucleus frequency in each of the patients studied. When the results for micronucleus frequency (Y) were plotted against the estimated equivalent whole-body dose (X) the dose-response relationship obtained was Y = 75·8X + 49·5 (r = 0·783, P < 0·0001). A general decline in MN frequency was observed during the post-treatment period down to 57 per cent (± 10) after 12 months but there was considerable variation between individuals. The advantages and disadvantages of the application of the cytokinesis-block micronucleus assay as a biological dosemeter for lymphocytes irradiated in vivo are discussed.

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Micronuclei in cytokinesis-blocked lymphocytes of cancer patients following fractionated partial-body radiotherapy

Abstract

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