MicroRNA signatures in chemotherapy resistant esophageal cancer cell lines

Richard Hummel, Corina Sie, David Watson, Tingting Wang, Alfiya Ansar, Michael Michael, Mark Van der Hoek, Joerg Haier, Damian Hussey

    Research output: Contribution to journalArticle

    48 Citations (Scopus)

    Abstract

    AIM: To investigate expression of microRNA (miRNA) and potential targets in chemotherapy resistant esophageal cancer cell lines. METHODS: An in-vitro model of acquired chemotherapy resistance in esophageal adeno- (EAC) and squamous cell carcinoma (ESCC) cells was used, and microRNA expression profiles for cisplatin or 5-fluorouracil (5-FU) resistant variants vs chemotherapy sensitive controls were compared using microarray and quantitative real-time polymerase chain reaction (PCR). The expression of chemotherapy-relevant genes potentially targeted by the dysregulated microRNAs in the chemotherapy resistant variants was also evaluated. RESULTS: Chemotherapy resistant sublines were found to have specific miRNA signatures, and these miRNA signatures were different for the cisplatin vs 5-FU resistant cells from the same tumor cell line, and also for EAC vs ESCC cells with resistance to the same specific chemotherapy agent. Amongst others, miR-27b-3p, miR-193b-3p, miR-192-5p, miR-378 a-3p, miR-125a-5p and miR-18a-3p were dysregulated, consistent with negative posttranscriptional control of KRAS, TYMS, ABCC3, CBL-B and ERBB2 expression via these miRNAs. CONCLUSION: The current study supports the hypothesis that microRNA expression has an impact on chemotherapy resistance in esophageal cancer.

    Original languageEnglish
    Pages (from-to)14904-14912
    Number of pages9
    JournalWorld Journal of Gastroenterology
    Volume20
    Issue number40
    DOIs
    Publication statusPublished - 2014

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