Microsatellite markers for the freshwater shrimp Paratya australiensis (Atyidae)

Douglas Green; Laurence Clarke; Duncan Mackay; Molly Whalen

doi:10.1007/s12686-010-9346-7

Microsatellite markers for the freshwater shrimp Paratya australiensis (Atyidae)

Douglas Green, Laurence Clarke, Duncan Mackay, Molly Whalen

Research output: Contribution to journal › Comment/debate

2 Citations (Scopus)

Abstract

Paratya australiensis is a common freshwater shrimp found in most catchments in south-eastern Australia. Microsatellite loci were isolated from a partial genomic library created using 454 sequencing. Of the 25 tested, 13 were found to be polymorphic,however, for pooling purposes only 12 were used for genotyping. The number of alleles per locus varied from 2 to 14 in a population from Wakefield River, South Australia, and the mean (range) observed and expected heterozygosity were 0.512 (0.136-0.909) and 0.590 (0.165-0.788), respectively across all alleles. These microsatellites will be used to study the genetic structure of populations in two catchments in South Australia.

Original language	English
Pages (from-to)	295-297
Number of pages	3
Journal	Conservation Genetics Resources
Volume	3
Issue number	2
DOIs	https://doi.org/10.1007/s12686-010-9346-7
Publication status	Published - Apr 2011

Keywords

454 sequencing
Freshwater shrimp
Macroinvertebrate
Microsatellites
Paratya
South Australia

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10.1007/s12686-010-9346-7

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title = "Microsatellite markers for the freshwater shrimp Paratya australiensis (Atyidae)",

abstract = "Paratya australiensis is a common freshwater shrimp found in most catchments in south-eastern Australia. Microsatellite loci were isolated from a partial genomic library created using 454 sequencing. Of the 25 tested, 13 were found to be polymorphic,however, for pooling purposes only 12 were used for genotyping. The number of alleles per locus varied from 2 to 14 in a population from Wakefield River, South Australia, and the mean (range) observed and expected heterozygosity were 0.512 (0.136-0.909) and 0.590 (0.165-0.788), respectively across all alleles. These microsatellites will be used to study the genetic structure of populations in two catchments in South Australia.",

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AU - Green, Douglas

AU - Clarke, Laurence

AU - Mackay, Duncan

AU - Whalen, Molly

PY - 2011/4

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N2 - Paratya australiensis is a common freshwater shrimp found in most catchments in south-eastern Australia. Microsatellite loci were isolated from a partial genomic library created using 454 sequencing. Of the 25 tested, 13 were found to be polymorphic,however, for pooling purposes only 12 were used for genotyping. The number of alleles per locus varied from 2 to 14 in a population from Wakefield River, South Australia, and the mean (range) observed and expected heterozygosity were 0.512 (0.136-0.909) and 0.590 (0.165-0.788), respectively across all alleles. These microsatellites will be used to study the genetic structure of populations in two catchments in South Australia.

AB - Paratya australiensis is a common freshwater shrimp found in most catchments in south-eastern Australia. Microsatellite loci were isolated from a partial genomic library created using 454 sequencing. Of the 25 tested, 13 were found to be polymorphic,however, for pooling purposes only 12 were used for genotyping. The number of alleles per locus varied from 2 to 14 in a population from Wakefield River, South Australia, and the mean (range) observed and expected heterozygosity were 0.512 (0.136-0.909) and 0.590 (0.165-0.788), respectively across all alleles. These microsatellites will be used to study the genetic structure of populations in two catchments in South Australia.

KW - 454 sequencing

KW - Freshwater shrimp

KW - Macroinvertebrate

KW - Microsatellites

KW - Paratya

KW - South Australia

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DO - 10.1007/s12686-010-9346-7

M3 - Comment/debate

SN - 1877-7252

VL - 3

SP - 295

EP - 297

JO - Conservation Genetics Resources

JF - Conservation Genetics Resources

IS - 2

ER -

Microsatellite markers for the freshwater shrimp Paratya australiensis (Atyidae)

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Keywords

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