TY - JOUR
T1 - Microstructured Optical Fiber-based Biosensors
T2 - Reversible and Nanoliter-Scale Measurement of Zinc Ions
AU - Heng, Sabrina
AU - McDevitt, Christopher A.
AU - Kostecki, Roman
AU - Morey, Jacqueline R.
AU - Eijkelkamp, Bart A.
AU - Ebendorff-Heidepriem, Heike
AU - Monro, Tanya M.
AU - Abell, Andrew D.
PY - 2016/5/13
Y1 - 2016/5/13
N2 - Sensing platforms that allow rapid and efficient detection of metal ions would have applications in disease diagnosis and study, as well as environmental sensing. Here, we report the first microstructured optical fiber-based biosensor for the reversible and nanoliter-scale measurement of metal ions. Specifically, a photoswitchable spiropyran Zn2+ sensor is incorporated within the microenvironment of a liposome attached to microstructured optical fibers (exposed-core and suspended-core microstructured optical fibers). Both fiber-based platforms retains high selectivity of ion binding associated with a small molecule sensor, while also allowing nanoliter volume sampling and on/off switching. We have demonstrated that multiple measurements can be made on a single sample without the need to change the sensor. The ability of the new sensing platform to sense Zn2+ in pleural lavage and nasopharynx of mice was compared to that of established ion sensing methodologies such as inductively coupled plasma mass spectrometry (ICP-MS) and a commercially available fluorophore (Fluozin-3), where the optical-fiber-based sensor provides a significant advantage in that it allows the use of nanoliter (nL) sampling when compared to ICP-MS (mL) and FluoZin-3 (μL). This work paves the way to a generic approach for developing surface-based ion sensors using a range of sensor molecules, which can be attached to a surface without the need for its chemical modification and presents an opportunity for the development of new and highly specific ion sensors for real time sensing applications.
AB - Sensing platforms that allow rapid and efficient detection of metal ions would have applications in disease diagnosis and study, as well as environmental sensing. Here, we report the first microstructured optical fiber-based biosensor for the reversible and nanoliter-scale measurement of metal ions. Specifically, a photoswitchable spiropyran Zn2+ sensor is incorporated within the microenvironment of a liposome attached to microstructured optical fibers (exposed-core and suspended-core microstructured optical fibers). Both fiber-based platforms retains high selectivity of ion binding associated with a small molecule sensor, while also allowing nanoliter volume sampling and on/off switching. We have demonstrated that multiple measurements can be made on a single sample without the need to change the sensor. The ability of the new sensing platform to sense Zn2+ in pleural lavage and nasopharynx of mice was compared to that of established ion sensing methodologies such as inductively coupled plasma mass spectrometry (ICP-MS) and a commercially available fluorophore (Fluozin-3), where the optical-fiber-based sensor provides a significant advantage in that it allows the use of nanoliter (nL) sampling when compared to ICP-MS (mL) and FluoZin-3 (μL). This work paves the way to a generic approach for developing surface-based ion sensors using a range of sensor molecules, which can be attached to a surface without the need for its chemical modification and presents an opportunity for the development of new and highly specific ion sensors for real time sensing applications.
KW - biosensor
KW - liposome
KW - microstructured optical fiber
KW - nanoscale
KW - photoswitch
KW - zinc
UR - http://www.scopus.com/inward/record.url?scp=84973345110&partnerID=8YFLogxK
UR - http://purl.org/au-research/grants/arc/CE140100003
UR - http://purl.org/au-research/grants/arc/DP150101856
U2 - 10.1021/acsami.6b03565
DO - 10.1021/acsami.6b03565
M3 - Article
C2 - 27152578
AN - SCOPUS:84973345110
SN - 1944-8244
VL - 8
SP - 12727
EP - 12732
JO - ACS Applied Materials and Interfaces
JF - ACS Applied Materials and Interfaces
IS - 20
ER -