miR-200 family expression is downregulated upon neoplastic progression of Barrett's esophagus

Cameron Smith, David Watson, Mary Leong, George Mayne, Michael Michael, Bas Wijnhoven, Damian Hussey

    Research output: Contribution to journalArticlepeer-review

    38 Citations (Scopus)

    Abstract

    AIM: To investigate miR-200 family expression in Barrett's epithelium, gastric and duodenal epithelia, and esophageal adenocarcinoma. METHODS: Real-time reverse transcriptase-polymerase chain reaction was used to measure miR-200, ZEB1 and ZEB2 expression. Ingenuity Pathway Analysis of miR-200 targets was used to predict biological outcomes. RESULTS: Barrett's epithelium expressed lower levels of miR-141 and miR-200c than did gastric and duodenal epithelia (P < 0.001). In silico analysis indicated roles for the miR-200 family in molecular pathways that distinguish Barrett's epithelium from gastric and duodenal epithelia, and which control apoptosis and proliferation. All miR-200 members were downregulated in adenocarcinoma (P < 0.02), and miR-200c expression was also downregulated in non-invasive epithelium adjacent to adenocarcinoma (P < 0.02). The expression of all miR-200 members was lower in Barrett's epithelium derived high-grade dysplastic cell lines than in a cell line derived from benign Barrett's epithelium. We observed significant inverse correlations between miR-200 family expression and ZEB1 and ZEB2 expression in Barrett's epithelium and esophageal adenocarcinoma (P < 0.05). CONCLUSION: miR-200 expression might contribute to the anti-apoptotic and proliferative phenotype of Barrett's epithelium and regulate key neoplastic processes in this epithelium.

    Original languageEnglish
    Pages (from-to)1036-1044
    Number of pages9
    JournalWorld Journal of Gastroenterology
    Volume17
    Issue number8
    DOIs
    Publication statusPublished - 28 Feb 2011

    Keywords

    • Apoptosis
    • Barrett's esophagus
    • Epithelial to mesenchymal transition
    • Epithelium
    • Esophageal adenocarcinoma
    • Mir-200
    • Mirna
    • Proliferation

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