Model Systems for Studying Mechanisms of Ocular Toxoplasmosis

Justine R. Smith, Liam M. Ashander, Yuefang Ma, Elise Rochet, João M. Furtado

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The most common human disease caused by infection with Toxoplasma gondii is ocular toxoplasmosis, which typically is manifest as recurrent attacks of necrotizing retinal inflammation with subsequent scarring. The multilayered retina contains specialized cell populations, including endothelial cells, epithelial cells, neurons and supporting cells, all of which may be involved in this condition. In vitro investigations of basic mechanisms operating in human ocular toxoplasmosis use cellular and molecular methods that are common to the study of many pathological processes, and the novel aspect of this research is the use of human retinal cell subsets. Most in vivo research on ocular toxoplasmosis is conducted in the laboratory mouse. Experimental models involve local or systemic inoculation of parasites to induce acute disease, or sequential systemic and local parasite inoculations to trigger recurrent disease. We present methods for in vitro and in vivo studies of ocular toxoplasmosis, including dissection of the human eye, and culture and infection of differentiated cell populations from the retina, as well as induction of mouse ocular toxoplasmosis by intraocular, or sequential systemic and intraocular, inoculations, and imaging of toxoplasmic retinal lesions.

Original languageEnglish
Title of host publicationToxoplasma gondii
Subtitle of host publicationMethods and Protocols
EditorsChristopher J Tonkin
Place of PublicationUnited States
PublisherHumana Press Inc.
Chapter17
Pages297-321
Number of pages25
ISBN (Electronic)978-1-4939-9857-9
ISBN (Print)978-1-4939-9856-2
DOIs
Publication statusPublished - 1 Jan 2020

Publication series

NameMethods in Molecular Biology
Volume2071
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Bibliographical note

Author version made available following 24 month embargo period per publisher's policy.

Keywords

  • Eye
  • Human
  • Mouse
  • Ocular toxoplasmosis
  • Retina
  • Toxoplasma

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    Smith, J. R., Ashander, L. M., Ma, Y., Rochet, E., & Furtado, J. M. (2020). Model Systems for Studying Mechanisms of Ocular Toxoplasmosis. In C. J. Tonkin (Ed.), Toxoplasma gondii: Methods and Protocols (pp. 297-321). (Methods in Molecular Biology; Vol. 2071). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9857-9_17