Modular Light-Emitting Diode Shelving Systems for Scalable Optogenetics

Christina G. Gangemi, Harald Janovjak

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

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Abstract

Optogenetic experiments rely on the controlled delivery of light to diverse biological systems. Impressive devices have been recently developed to stimulate cells and small animals with multiple wavelengths and intensities. However, existing hardware solutions are often limited to a single sample holder, and their design and cost can further limit scalability. This chapter describes an illumination system that is modular (through the use of accessible components) and scalable (through a shelving structure and low cost). Assembly and operation require no or minimal electrical engineering or programming expertise. Multi-intensity wavelength and temporal experiments can be performed in dozens of small and large samples. This chapter also introduces methods for temperature and light intensity measurements toward appropriate illumination conditions. This work aims to provide a greater level of accessibility and complementary opportunities for large-scale optogenetics in a broad range of biological samples.

Original languageEnglish
Title of host publicationOptogenetics
Subtitle of host publicationMethods and Protocols
EditorsArmin Baumschlager
PublisherSpringer Nature
Chapter17
Pages231-244
Number of pages14
Volume2840
ISBN (Electronic)978-1-0716-4047-0
ISBN (Print)978-1-0716-4046-3
DOIs
Publication statusPublished - 2025

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Number2840
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Illumination hardware
  • LED
  • Optical control
  • Optics
  • Optogenetics
  • Synthetic biology

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