TY - JOUR
T1 - Molecular characterization and enzymatic hydrolysis of naringin extracted from kinnow peel waste
AU - Puri, Munish
AU - Kaur, Aneet
AU - Schwarz, Wolfgang
AU - Singh, Satbir
AU - Kennedy, J. F.
PY - 2011/1/1
Y1 - 2011/1/1
N2 - Kinnow peel, a waste rich in glycosylated phenolic substances, is the principal by-product of the citrus fruit processing industry and its disposal is becoming a major problem. This peel is rich in naringin and may be used for rhamnose production by utilizing α-l-rhamnosidase (EC 3.2.1.40), an enzyme that catalyzes the cleavage of terminal rhamnosyl groups from naringin to yield prunin and rhamnose. In this work, infrared (IR) spectroscopy confirmed molecular characteristics of naringin extracted from kinnow peel waste. Further, recombinant α-l-rhamnosidase purified from Escherichia coli cells using immobilized metal-chelate affinity chromatography (IMAC) was used for naringin hydrolysis. The purified enzyme was inhibited by Hg2+ (1mM), 4-hydroxymercuribenzoate (0.1mM) and cyanamide (0.1mM). The purified enzyme established hydrolysis of naringin extracted from kinnow peel and thus endorses its industrial applicability for producing rhamnose.
AB - Kinnow peel, a waste rich in glycosylated phenolic substances, is the principal by-product of the citrus fruit processing industry and its disposal is becoming a major problem. This peel is rich in naringin and may be used for rhamnose production by utilizing α-l-rhamnosidase (EC 3.2.1.40), an enzyme that catalyzes the cleavage of terminal rhamnosyl groups from naringin to yield prunin and rhamnose. In this work, infrared (IR) spectroscopy confirmed molecular characteristics of naringin extracted from kinnow peel waste. Further, recombinant α-l-rhamnosidase purified from Escherichia coli cells using immobilized metal-chelate affinity chromatography (IMAC) was used for naringin hydrolysis. The purified enzyme was inhibited by Hg2+ (1mM), 4-hydroxymercuribenzoate (0.1mM) and cyanamide (0.1mM). The purified enzyme established hydrolysis of naringin extracted from kinnow peel and thus endorses its industrial applicability for producing rhamnose.
KW - Citrus
KW - IMAC
KW - Infrared spectroscopy
KW - Inhibitor
KW - Naringin
KW - Recombinant rhamnosidase
UR - http://www.scopus.com/inward/record.url?scp=78650230116&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2010.09.012
DO - 10.1016/j.ijbiomac.2010.09.012
M3 - Article
VL - 48
SP - 58
EP - 62
JO - INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
JF - INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
SN - 0141-8130
IS - 1
ER -