Molecular characterization of a cytosolic manganese superoxide dismutase from the Chinese mitten crab Eriocheir sinensis

Daxiang Zhao, Liqiao Chen, Jianguang Qin, Chuanjie Qin, Hao Zhang, Ping Wu, Erchao Li

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    8 Citations (Scopus)

    Abstract

    A cytosolic manganese superoxide dismutase gene (EscMnSOD) was cloned from the Chinese mitten crab Eriocheir sinensis, using reverse transcription-polymerase chain reaction and the rapid amplification of cDNA ends. The open reading frame of Es-cMnSOD is 867 bp in length and encodes a 288-amino acid protein without a signal peptide. The calculated molecular mass of the translated protein of Es-cMnSOD is 31.43 kDa, with an estimated isoelectric point of 6.30. The deduced amino acid sequence of Es-cMnSOD has similarities of 90, 89, 84, 87, and 81% to those of white shrimp Litopenaeus vannamei MnSOD, black tiger shrimp Penaeus monodon MnSOD, giant freshwater prawn Macrobrachium rosenbergii MnSOD, blue crab Callinectes sapidus MnSOD, and red swamp crayfish Procambarus clarkii MnSOD, respectively. Es-cMnSOD contains a manganese superoxide dismutase domain (DVWEHAYY) and 4 conserved amino acids responsible for binding manganese. Es-cMnSOD was expressed in the hemocytes, eyestalk, muscle, intestine, gill, and hepatopancreas. Es-cMnSOD transcripts in hemocytes of E. sinensis increased at 1.5 and 48 h after injection of Aeromonas hydrophila, indicating that the induction of the SOD system response occurred within a short period of time. This study suggests that MnSOD may play a critical role in crab immunity, allowing efficient activation of an early innate immune response in the crab.

    Original languageEnglish
    Pages (from-to)9429-9442
    Number of pages14
    JournalGENETICS AND MOLECULAR RESEARCH
    Volume13
    Issue number4
    DOIs
    Publication statusPublished - 11 Nov 2014

    Keywords

    • Aeromonas hydrophila
    • Cytosolic manganese superoxide dismutase
    • Eriocheir sinensis
    • Hemocyte

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