Molecular Characterization of Nanoimmobilized Cellulase in Facilitating Pretreatment of Lignocellulosic Biomass

Reinu E. Abraham, Colin J. Barrow, Munish Puri

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

2 Citations (Scopus)


Biofuel production from biomass requires an effective bioprocessing of lignocellulosic biomass. It involves pretreatment of biomass, a synergistic action of cellulase to break the complex structure, and release fermentable sugars from hydrolyzed biomass. The yield of the sugar hydrolysate crucially depends on the efficiency of enzymatic hydrolysis. An inefficient pretreatment may cause low concentration of sugars, excessive addition of enzymes, and high production of inhibitors. These consequences and the high cost of the enzyme make the process expensive. An immobilization system allows the covalent attachment of enzymes on a magnetic or nonmagnetic support, which improves the biochemical properties of the respective enzyme. It makes the enzyme stable, reusable, and provides easier separation from the reaction mixture. The enzyme attains thermostability and storage stability properties.

Original languageEnglish
Title of host publicationNew and Future Developments in Microbial Biotechnology and Bioengineering
Subtitle of host publicationMicrobial Cellulase System Properties and Applications
EditorsVijai Kumar Gupta
Place of PublicationNetherlands
PublisherElsevier Inc.
Number of pages9
ISBN (Electronic)9780444635150
ISBN (Print)9780444635075
Publication statusPublished - 2016
Externally publishedYes


  • Covalent binding, reusability
  • Immobilization
  • Lignocellulose
  • Nanomaterial


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