Molecular Cloning and Chromosomal Mapping of the Human Homologue of MYB Binding Protein (P160) 1A (MYBBP1A) to 17p13.3

Rebecca Keough; Erica Woollatt; Joanna Crawford; Grant R. Sutherland; Sarah Plummer; Graham Casey; Thomas J. Gonda

doi:10.1006/geno.1999.6035

Molecular Cloning and Chromosomal Mapping of the Human Homologue of MYB Binding Protein (P160) 1A (MYBBP1A) to 17p13.3

Rebecca Keough, Erica Woollatt, Joanna Crawford, Grant R. Sutherland, Sarah Plummer, Graham Casey, Thomas J. Gonda

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Abstract

We have previously isolated and characterized murine MYB binding protein (p160) la, a protein that specifically interacts with the leucine zipper motif within the negative regulatory domain of the c-Myb proto-oncoprotein. We now describe the molecular cloning of the human MYBBP1A cDNA and chromosomal localization to 17p13.3 by fluorescence in situ hybridization analysis. Given the likely presence of a tumor suppressor gene (or genes) within this region of chromosome 17, the position of MYBBP1A was further mapped by radiation hybrid analysis and was found to lie between markers D17S1828 and D17S938. A P1 artificial chromosome clone containing the 5' region of MYBBP1A was isolated and indicates a physical linkage between MYBBP1A and the 15-lipoxygenase gene (ALOX15). A novel, polymorphic (CA)₂₅ dinucleotide repeat was also isolated from this PAC and may serve as a useful marker for MYBBP1A and this region of chromosome 17. (C) 1999 Academic Press.

Original language	English
Pages (from-to)	483-489
Number of pages	7
Journal	Genomics
Volume	62
Issue number	3
DOIs	https://doi.org/10.1006/geno.1999.6035
Publication status	Published - 15 Dec 1999
Externally published	Yes

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title = "Molecular Cloning and Chromosomal Mapping of the Human Homologue of MYB Binding Protein (P160) 1A (MYBBP1A) to 17p13.3",

abstract = "We have previously isolated and characterized murine MYB binding protein (p160) la, a protein that specifically interacts with the leucine zipper motif within the negative regulatory domain of the c-Myb proto-oncoprotein. We now describe the molecular cloning of the human MYBBP1A cDNA and chromosomal localization to 17p13.3 by fluorescence in situ hybridization analysis. Given the likely presence of a tumor suppressor gene (or genes) within this region of chromosome 17, the position of MYBBP1A was further mapped by radiation hybrid analysis and was found to lie between markers D17S1828 and D17S938. A P1 artificial chromosome clone containing the 5' region of MYBBP1A was isolated and indicates a physical linkage between MYBBP1A and the 15-lipoxygenase gene (ALOX15). A novel, polymorphic (CA)25 dinucleotide repeat was also isolated from this PAC and may serve as a useful marker for MYBBP1A and this region of chromosome 17. (C) 1999 Academic Press.",

author = "Rebecca Keough and Erica Woollatt and Joanna Crawford and Sutherland, {Grant R.} and Sarah Plummer and Graham Casey and Gonda, {Thomas J.}",

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T1 - Molecular Cloning and Chromosomal Mapping of the Human Homologue of MYB Binding Protein (P160) 1A (MYBBP1A) to 17p13.3

AU - Keough, Rebecca

AU - Woollatt, Erica

AU - Crawford, Joanna

AU - Sutherland, Grant R.

AU - Plummer, Sarah

AU - Casey, Graham

AU - Gonda, Thomas J.

PY - 1999/12/15

Y1 - 1999/12/15

N2 - We have previously isolated and characterized murine MYB binding protein (p160) la, a protein that specifically interacts with the leucine zipper motif within the negative regulatory domain of the c-Myb proto-oncoprotein. We now describe the molecular cloning of the human MYBBP1A cDNA and chromosomal localization to 17p13.3 by fluorescence in situ hybridization analysis. Given the likely presence of a tumor suppressor gene (or genes) within this region of chromosome 17, the position of MYBBP1A was further mapped by radiation hybrid analysis and was found to lie between markers D17S1828 and D17S938. A P1 artificial chromosome clone containing the 5' region of MYBBP1A was isolated and indicates a physical linkage between MYBBP1A and the 15-lipoxygenase gene (ALOX15). A novel, polymorphic (CA)25 dinucleotide repeat was also isolated from this PAC and may serve as a useful marker for MYBBP1A and this region of chromosome 17. (C) 1999 Academic Press.

AB - We have previously isolated and characterized murine MYB binding protein (p160) la, a protein that specifically interacts with the leucine zipper motif within the negative regulatory domain of the c-Myb proto-oncoprotein. We now describe the molecular cloning of the human MYBBP1A cDNA and chromosomal localization to 17p13.3 by fluorescence in situ hybridization analysis. Given the likely presence of a tumor suppressor gene (or genes) within this region of chromosome 17, the position of MYBBP1A was further mapped by radiation hybrid analysis and was found to lie between markers D17S1828 and D17S938. A P1 artificial chromosome clone containing the 5' region of MYBBP1A was isolated and indicates a physical linkage between MYBBP1A and the 15-lipoxygenase gene (ALOX15). A novel, polymorphic (CA)25 dinucleotide repeat was also isolated from this PAC and may serve as a useful marker for MYBBP1A and this region of chromosome 17. (C) 1999 Academic Press.

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SN - 0888-7543

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JF - Genomics

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Molecular Cloning and Chromosomal Mapping of the Human Homologue of MYB Binding Protein (P160) 1A (MYBBP1A) to 17p13.3

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