Molecular cloning, characterization, and mRNA expression of hemocyanin subunit in oriental river prawn Macrobrachium nipponense

Youqin Kong, Liqiao Chen, Zhili Ding, Jianguang Qin, Shengming Sun, Ligai Wang, Jinyun Ye

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    Abstract

    Hemocyanin is a copper-containing protein with immune function against disease. In this study, a hemocyanin subunit named MnHc-1 was cloned from Macrobrachium nipponense. The full-length cDNA of MnHc-1 was 2,163 bp with a 2,028-bp open reading frame (ORF) encoding a polypeptide of 675 amino acids. The MnHc-1 mRNA was expressed in the hepatopancreas, gill, hemocytes, intestine, ovary, and stomach, with the highest level in the hepatopancreas. In the infection trial, the MnHc-1 mRNA transcripts in the hemocytes were significantly downregulated at 3 h after injection of Aeromonas hydrophila and then upregulated at 6 h and 12 h, followed by a gradual recovery from 24 to 48 h. The MnHc-1 transcriptional expression in the hepatopancreas was measured after M. nipponense were fed seven diets with 2.8, 12.2, 20.9, 29.8, 43.1, 78.9, and 157.1 mg Cu kg -1 for 8 weeks, respectively. The level of MnHc-1 mRNA was significantly higher in the prawns fed 43.1-157.1 mg Cu kg -1 diet than in that fed 2.8-29.8 mg Cu kg -1 diet. This study indicated that the MnHc-1 expression can be affected by dietary copper and the hemocyanin may potentially participate in the antibacterial defense of M. nipponense.

    Original languageEnglish
    Article number6404817
    Pages (from-to)Art: 6404817
    Number of pages9
    JournalInternational Journal of Genomics
    Volume2016
    DOIs
    Publication statusPublished - 2016

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