Molecular Signals Involved in Human B Cell Migration into the Retina: In Vitro Investigation of ICAM-1, VCAM-1, and CXCL13

Arpita Bharadwaj, Andrew Stempel, Antoinette Olivas, Samone Franzese, Liam Ashander, Yuefang Ma, Shervi Lie, Binoy Appukuttan, Justine Smith

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Purpose: B cells participate in diverse retinal immunopathologies. Endothelial adhesion molecules and chemokines direct leukocyte trafficking. We examined the involvement of three molecular signals in retinal transendothelial migration of human B cells: ICAM-1, VCAM-1, and CXCL13. Methods: Peripheral blood B cells were isolated by negative selection. Migration was studied in transwells populated with human retinal endothelial monolayers, using antibody to block ICAM-1 or VCAM-1. Retinal expression of CXCL13 was investigated. Results: B cells crossed retinal endothelium. ICAM-1 blockade significantly reduced migration when results for all subjects were combined, and for a majority when results were analyzed by individual. This effect was irrespective of the presence or absence of CXCL13, although CXCL13 increased migration. CXCL13 was detected in neural retina and retinal pigment epithelium. Endothelial cells of some retinal vessels presented CXCL13 protein. Conclusion: ICAM-1 blockade may be an effective treatment in some patients with retinal diseases that involve B cells.

Original languageEnglish
Pages (from-to)811-819
Number of pages9
JournalOcular Immunology and Inflammation
Volume25
Issue number6
DOIs
Publication statusPublished - 2017

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