Molecular weight analysis of soluble antigens from Toxoplasma gondii

A. M. Johnson, P. J. McDonald, S. H. Neoh

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    Abstract

    Ultrasonicated Toxoplasma gondii (RH strain) tachyzoites were fractionated into a water-soluble and a deoxycholate-soluble fraction. Polyclonal immune mouse serum was prepared by challenging chronically-infected mice with viable RH strain tachyzoites. The parasite functions were labelled with 125I, and the radiolabelled antigens were precipitated by the immune mouse serum or a monoclonal anti-Toxoplasma antibody (FMC 20), that reacts only in the indirect hemagglutination antibody test. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography of the immunoprecipitates showed that the water-soluble fraction contained 10 antigenic polypeptides, and the deoxycholate-soluble fraction contained seven antigenic peptides. The FMC 20 reacted against a 98,000-dalton antigen that was present in the water-soluble fraction only.

    Original languageEnglish
    Pages (from-to)459-464
    Number of pages6
    JournalJournal of Parasitology
    Volume69
    Issue number3
    DOIs
    Publication statusPublished - Jun 1983

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