Molecular weight analysis of soluble antigens from Toxoplasma gondii

A. M. Johnson; P. J. McDonald; S. H. Neoh

doi:10.2307/3281354

Molecular weight analysis of soluble antigens from Toxoplasma gondii

A. M. Johnson, P. J. McDonald, S. H. Neoh

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15 Citations (Scopus)

Abstract

Ultrasonicated Toxoplasma gondii (RH strain) tachyzoites were fractionated into a water-soluble and a deoxycholate-soluble fraction. Polyclonal immune mouse serum was prepared by challenging chronically-infected mice with viable RH strain tachyzoites. The parasite functions were labelled with ¹²⁵I, and the radiolabelled antigens were precipitated by the immune mouse serum or a monoclonal anti-Toxoplasma antibody (FMC 20), that reacts only in the indirect hemagglutination antibody test. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography of the immunoprecipitates showed that the water-soluble fraction contained 10 antigenic polypeptides, and the deoxycholate-soluble fraction contained seven antigenic peptides. The FMC 20 reacted against a 98,000-dalton antigen that was present in the water-soluble fraction only.

Original language	English
Pages (from-to)	459-464
Number of pages	6
Journal	Journal of Parasitology
Volume	69
Issue number	3
DOIs	https://doi.org/10.2307/3281354
Publication status	Published - Jun 1983

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abstract = "Ultrasonicated Toxoplasma gondii (RH strain) tachyzoites were fractionated into a water-soluble and a deoxycholate-soluble fraction. Polyclonal immune mouse serum was prepared by challenging chronically-infected mice with viable RH strain tachyzoites. The parasite functions were labelled with 125I, and the radiolabelled antigens were precipitated by the immune mouse serum or a monoclonal anti-Toxoplasma antibody (FMC 20), that reacts only in the indirect hemagglutination antibody test. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography of the immunoprecipitates showed that the water-soluble fraction contained 10 antigenic polypeptides, and the deoxycholate-soluble fraction contained seven antigenic peptides. The FMC 20 reacted against a 98,000-dalton antigen that was present in the water-soluble fraction only.",

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AU - McDonald, P. J.

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