Molecular weight analysis of the major polypeptides and glycopeptides of Toxoplasma gondii

Alan M. Johnson, P. J. McDonald, S. H. Neoh

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Abstract

A soluble and an insoluble fraction of ultrasonicated Toxoplasma gondii (RH strain) tachyzoites were prepared by ultracentrifugation. The insoluble parasite fraction was solubilized by incubation in 1% sodium deoxycholate (DOC). The molecular weights (m.w.) of the polypeptides in the fractions were determined by sodium dodecyl sulphate (SDS) - polyacrylamide gel electrophoresis (PAGE). After comparison with the m.w. of the polypeptides of appropriate controls the soluble Toxoplasma fraction contained at least 9 parasite polypeptides with m.w. of 20K, 22K, 23K, 29K, 66K, 69K, 90K, 98K and 133K and the insoluble parasite fraction contained at least 8 Toxoplasma derived polypeptides with m.w. of 28K, 41K, 43K, 49K, 53K, 61K, 70K and 80K. The presence of glycosylated polypeptides was detected by incubation of sliced gels with |125I| Concanavalin A (Con A). The soluble Toxoplasma fraction contained no major glycopeptide bands while the insoluble parasite fraction contained at least 3 glycosylated polypeptides of Toxoplasma origin with m.w. of 29K, 53K and 123K.

Original languageEnglish
Pages (from-to)934-943
Number of pages10
JournalBiochemical and Biophysical Research Communications
Volume100
Issue number3
DOIs
Publication statusPublished - 16 Jun 1981
Externally publishedYes

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