Molting, tissue calcium‑phosphorus deposition and immunity of juvenile Chinese mitten crab (Eriocheir sinensis) fed different levels of calcium and vitamin D3

Shubin Liu, Xinyu Li, Xiaodan Wang, Xianyong Bu, Erchao Li, Meiling Zhang, Chuanjie Qin, Jian G. Qin, Liqiao Chen

Research output: Contribution to journalArticlepeer-review

Abstract

Molting activity and innate immunity play a pivotal role in the development and growth of crustaceans and are related to mortality and body abnormality. To evaluate the synergistic effect of vitamin D3 (vit-D3) and Ca in molting and immunity in Chinese mitten crab (Eriocheir sinensis), the crabs (3.20 ± 0.01 g) were fed six diets formulated with two Ca levels (0% and 1.5%, abbreviated D and N) and three vit-D3 levels (0, 9,000 and 27,000 IU/kg, abbreviated LVD, MVD and HVD) level for 56 days. Crabs fed 1.5% Ca and 9,000 IU/kg vit-D3 (N + MVD) had the highest growth performance, hematic-1,25-dihydroxy vit-D3, hematic‑calcium, hepatic‑phosphorus and antioxidant capacity among all groups. On the contrary, crabs fed diets without Ca reduced growth performance, tissue calcium‑phosphorus deposition, antioxidant capacity, and the expression of molting-related and immune-related genes. Compared to crab fed N + HVD (1.5% Ca + 27,000 IU/kg vit-D3), those fed D + HVD (0% Ca +27,000 IU/kg vit-D3) had significantly low growth performance and the gene expressions of molting and antibacterial immunity. This study demonstrates that adequate dietary calcium and vit-D3 can enhance growth, molting activity, antioxidant capacity and innate immunity in E. sinensis. Moreover, it provides an in-depth understanding of the molecular mechanism of molting activity and antibacterial immunity in Chinese mitten crab

Original languageEnglish
Article number738124
Number of pages11
JournalAquaculture
Volume554
DOIs
Publication statusPublished - 30 May 2022

Keywords

  • Calcium
  • Eriocheir sinensis
  • Immunity
  • Molting
  • Vitamin D

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