Monitoring of geosmin producing Anabaena circinalis using quantitative PCR

Hsiangwei Tsao, Atsuko Michinaka, Hungkai Yen, Steven Giglio, P Hobson, Paul Monis, Tsairfuh Lin

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    23 Citations (Scopus)

    Abstract

    Geosmin is one of the most commonly detected off-flavor chemicals present in reservoirs and drinking water systems. Quantitative real-time PCR (qPCR) is useful for quantifying geosmin-producers by focusing on the gene encoding geosmin synthase, which is responsible for geosmin synthesis. In this study, several primers and probes were designed and evaluated to detect the geosmin synthase gene in cyanobacteria. The specificity of primer and probe sets was tested using 21 strains of laboratory cultured cyanobacteria isolated from surface waters in Australia (18) and Taiwan (2), including 6 strains with geosmin producing ability. The results showed that the primers designed in this study could successfully detect all geosmin producing strains tested. The selected primers were used in a qPCR assay, and the calibration curves were linear from 5×101 to 5×105copiesmL-1, with a high correlation coefficient (R2=0.999). This method was then applied to analyze samples taken from Myponga Reservoir, South Australia, during a cyanobacterial bloom event. The results showed good correlations between qPCR techniques and traditional methods, including cell counts determined by microscopy and geosmin concentration measured using gas chromatography (GC) coupled with a mass selective detector (MSD). Results demonstrate that qPCR could be used for tracking geosmin-producing cyanobacteria in drinking water reservoirs. The qPCR assay may provide water utilities with the ability to properly characterize a taste and odor episode and choose appropriate management and treatment options.

    Original languageEnglish
    Pages (from-to)416-425
    Number of pages10
    JournalWater Research
    Volume49
    DOIs
    Publication statusPublished - 2014

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    Tsao, H., Michinaka, A., Yen, H., Giglio, S., Hobson, P., Monis, P., & Lin, T. (2014). Monitoring of geosmin producing Anabaena circinalis using quantitative PCR. Water Research, 49, 416-425. https://doi.org/10.1016/j.watres.2013.10.028