Monoclonality in B-lymphoproliferative disorders detected at the DNA level

K. J. Trainor; M. J. Brisco; C. J. Story; A. A. Morley

doi:10.1182/blood.V75.11.2220.2220

Monoclonality in B-lymphoproliferative disorders detected at the DNA level

K. J. Trainor, M. J. Brisco, C. J. Story, A. A. Morley

Research output: Contribution to journal › Article › peer-review

271 Citations (Scopus)

Abstract

A new method was developed for detection of monoclonality at the DNA level in B-lymphoproliferative disease using the polymerase chain reaction and consensus primers for the V and J regions of the immunoglobulin gene. Monoclonality was detected in DNA from 15 of 15 clonal B-lymphoblastoid cell lines and from 19 of 23 cases of B-lymphocyte neoplasia, but not from any of 16 normal T-lymphocyte clones, 9 cases of T-lymphocyte neoplasia, 20 samples of polyclonal peripheral blood lymphocytes, and 8 cases of reactive lymphadenopathy. This method for detection of monoclonality is likely to be of routine value in diagnosis owing to its simplicity, speed, and versatility.

Original language	English
Pages (from-to)	2220-2222
Number of pages	3
Journal	Blood
Volume	75
Issue number	11
DOIs	https://doi.org/10.1182/blood.V75.11.2220.2220
Publication status	Published - 1 Jun 1990
Externally published	Yes

Access to Document

10.1182/blood.V75.11.2220.2220Licence: In Copyright

Cite this

@article{9b89b13f71cc4e1f843ae7a4ebda2500,

title = "Monoclonality in B-lymphoproliferative disorders detected at the DNA level",

abstract = "A new method was developed for detection of monoclonality at the DNA level in B-lymphoproliferative disease using the polymerase chain reaction and consensus primers for the V and J regions of the immunoglobulin gene. Monoclonality was detected in DNA from 15 of 15 clonal B-lymphoblastoid cell lines and from 19 of 23 cases of B-lymphocyte neoplasia, but not from any of 16 normal T-lymphocyte clones, 9 cases of T-lymphocyte neoplasia, 20 samples of polyclonal peripheral blood lymphocytes, and 8 cases of reactive lymphadenopathy. This method for detection of monoclonality is likely to be of routine value in diagnosis owing to its simplicity, speed, and versatility.",

author = "Trainor, {K. J.} and Brisco, {M. J.} and Story, {C. J.} and Morley, {A. A.}",

year = "1990",

month = jun,

day = "1",

doi = "10.1182/blood.V75.11.2220.2220",

language = "English",

volume = "75",

pages = "2220--2222",

journal = "Blood",

issn = "0006-4971",

publisher = "Elsevier B.V.",

number = "11",

}

TY - JOUR

T1 - Monoclonality in B-lymphoproliferative disorders detected at the DNA level

AU - Trainor, K. J.

AU - Brisco, M. J.

AU - Story, C. J.

AU - Morley, A. A.

PY - 1990/6/1

Y1 - 1990/6/1

N2 - A new method was developed for detection of monoclonality at the DNA level in B-lymphoproliferative disease using the polymerase chain reaction and consensus primers for the V and J regions of the immunoglobulin gene. Monoclonality was detected in DNA from 15 of 15 clonal B-lymphoblastoid cell lines and from 19 of 23 cases of B-lymphocyte neoplasia, but not from any of 16 normal T-lymphocyte clones, 9 cases of T-lymphocyte neoplasia, 20 samples of polyclonal peripheral blood lymphocytes, and 8 cases of reactive lymphadenopathy. This method for detection of monoclonality is likely to be of routine value in diagnosis owing to its simplicity, speed, and versatility.

AB - A new method was developed for detection of monoclonality at the DNA level in B-lymphoproliferative disease using the polymerase chain reaction and consensus primers for the V and J regions of the immunoglobulin gene. Monoclonality was detected in DNA from 15 of 15 clonal B-lymphoblastoid cell lines and from 19 of 23 cases of B-lymphocyte neoplasia, but not from any of 16 normal T-lymphocyte clones, 9 cases of T-lymphocyte neoplasia, 20 samples of polyclonal peripheral blood lymphocytes, and 8 cases of reactive lymphadenopathy. This method for detection of monoclonality is likely to be of routine value in diagnosis owing to its simplicity, speed, and versatility.

UR - http://www.scopus.com/inward/record.url?scp=0025346475&partnerID=8YFLogxK

U2 - 10.1182/blood.V75.11.2220.2220

DO - 10.1182/blood.V75.11.2220.2220

M3 - Article

C2 - 2112032

AN - SCOPUS:0025346475

SN - 0006-4971

VL - 75

SP - 2220

EP - 2222

JO - Blood

JF - Blood

IS - 11

ER -

Monoclonality in B-lymphoproliferative disorders detected at the DNA level

Abstract

Access to Document

Other files and links

Fingerprint

Cite this