Monoclonality in B-lymphoproliferative disorders detected at the DNA level

K. J. Trainor, M. J. Brisco, C. J. Story, A. A. Morley

Research output: Contribution to journalArticlepeer-review

255 Citations (Scopus)


A new method was developed for detection of monoclonality at the DNA level in B-lymphoproliferative disease using the polymerase chain reaction and consensus primers for the V and J regions of the immunoglobulin gene. Monoclonality was detected in DNA from 15 of 15 clonal B-lymphoblastoid cell lines and from 19 of 23 cases of B-lymphocyte neoplasia, but not from any of 16 normal T-lymphocyte clones, 9 cases of T-lymphocyte neoplasia, 20 samples of polyclonal peripheral blood lymphocytes, and 8 cases of reactive lymphadenopathy. This method for detection of monoclonality is likely to be of routine value in diagnosis owing to its simplicity, speed, and versatility.

Original languageEnglish
Pages (from-to)2220-2222
Number of pages3
Issue number11
Publication statusPublished - 1 Jun 1990
Externally publishedYes


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