Monosodium urate crystal-induced prostaglandin synthesis in the rat subcutaneous air pouch

T. P. Gordon, I. C. Kowanko, M. James, P. J. Roberts-Thomson

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Monosodium urate crystal-induced inflammation was studied in an air pouch model of rat synovium. Phagocytosis of crystals by macrophage-like and fibroblast-like cells in the pouch lining was observed 30 minutes after crystal injection and preceded polymorphonuclear leukocyte accumulation. Levels of the prostaglandins PGE2 and 6-keto-PGF(1α), measured in serial pouch washouts, showed a clear temporal dissociation with leucocyte numbers, with peak levels of the prostanoids occurring before the maximum accumulation of leucocytes. Indomethacin abrogated prostaglandin synthesis completely without reducing leucocyte counts in pouch washouts. We propose that the pouch lining cells may be the primary source of the prostaglandins and these mediators do not have a significant role in leucocyte chemotaxis in this model.

Original languageEnglish
Pages (from-to)291-296
Number of pages6
JournalClinical and Experimental Rheumatology
Volume3
Issue number4
Publication statusPublished - 1985
Externally publishedYes

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