Morphological cell transformation of Syrian hamster embryo (SHE) cells by the cyanotoxin, cylindrospermopsin

Marie Maire, Emmanuelle Bazin, Valerie Fessard, Claudine Rast, Andrew Humpage, Paule Vasseur

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    24 Citations (Scopus)

    Abstract

    Cylindrospermopsin (CYN) is a cyanotoxin which has been implicated in human intoxication and animal mortality. Genotoxic activity of this hepatotoxin is known but its carcinogenic activity remains to be elucidated. In this work, CYN was assessed for its cell-transforming activity using the Syrian hamster embryo (SHE) cell transformation assay. This in vitro assay is used to evaluate the carcinogenic potential of chemical, physical and biological agents in SHE cells, which are primary, normal, diploid, genetically stable and capable of metabolic activation. We demonstrated that CYN induced a significant increase in morphological cell transformation in SHE cells following a 7-day continuous treatment in the range of non-cytotoxic concentrations 1 × 10-7-1 × 10-2 ng/mL.

    Original languageEnglish
    Pages (from-to)1317-1322
    Number of pages6
    JournalToxicon
    Volume55
    Issue number7
    DOIs
    Publication statusPublished - Jun 2010

    Keywords

    • Carcinogenicity
    • Cell transformation
    • Cyanotoxin
    • Cylindrospermopsin (CYN)
    • Low-dose effects
    • Syrian hamster embryo (SHE)

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