Multiple immunohistochemical labelling of peripheral neurons

    Research output: Chapter in Book/Report/Conference proceedingChapter

    Abstract

    This chapter describes well-established procedures for multiple-labelling immuno fl uorescence as applied to peripheral neurons. Tissues are fi xed with a mixture of formaldehyde and picric acid, and then processed through solvents (ethanol and dimethyl sulfoxide or xylene) before preparation as sections (frozen or embedded in polyethylene glycol) or whole mounts. Specimens are exposed to small volumes of antibody mixtures and are then mounted in buffered glycerol prior to examination with fl uorescent microscope fi tted with appropriate optics for multi-labelling fl uorescence. Critical controls are summarised for all stages of the process, including the speci fi city of the primary antibodies, the secondary antibodies, and the subsequent image acquisition and analysis

    Original languageEnglish
    Title of host publicationNeuromethods
    Subtitle of host publicationVisualization Techniques: From Immunohistochemistry to Magnetic Resonance Imaging
    EditorsE. Badoer
    PublisherHumana Press Inc.
    Pages1-30
    Number of pages30
    Volume70
    ISBN (Electronic)978-1-61779-897-9
    ISBN (Print)978-1-61779-896-2
    DOIs
    Publication statusPublished - 2012

    Publication series

    NameNeuromethods
    Volume70
    ISSN (Print)0893-2336
    ISSN (Electronic)1940-6045

    Keywords

    • Co-localisation
    • Confocal microscopy
    • Fluorescence microscopy
    • Immuno fluorescence
    • Multiple labelling
    • Peripheral neurons

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  • Cite this

    Gibbins, I. L. (2012). Multiple immunohistochemical labelling of peripheral neurons. In E. Badoer (Ed.), Neuromethods: Visualization Techniques: From Immunohistochemistry to Magnetic Resonance Imaging (Vol. 70, pp. 1-30). (Neuromethods; Vol. 70). Humana Press Inc.. https://doi.org/10.1007/978-1-61779-897-9_1