TY - JOUR
T1 - Multiple-response optimization of the acidic treatment of the brown alga Ecklonia radiata for the sequential extraction of fucoidan and alginate
AU - Lorbeer, Andrew
AU - Lahnstein, Jelle
AU - Bulone, Vincent
AU - Nguyen Thanh, Trung
AU - Zhang, Wei
PY - 2015/12/1
Y1 - 2015/12/1
N2 - The aim of this study was to optimize the acidic treatment of the brown alga Ecklonia radiata in order to extract fucoidan and facilitate the efficient sequential extraction of alginates. Response surface methodology was used to determine the effects of the temperature, pH, and duration of the acidic treatment on fucoidan yield, alginate extractability, and the molecular weight of sequentially extracted alginates. Desirability functions were then used to predict the best overall combinations of responses. The most desirable compromise allowed for the recovery of a fucoidan-rich fraction with a yield of 3.75% (w/w of alga) and the sequential extraction of alginates having an average molecular weight of 730. kDa at a yield of 44% (w/w of alga), with low cross-contamination between the products. The optimized acidic treatment could form the basis of an industrial biorefinery process for the production of both fucoidan and alginate.
AB - The aim of this study was to optimize the acidic treatment of the brown alga Ecklonia radiata in order to extract fucoidan and facilitate the efficient sequential extraction of alginates. Response surface methodology was used to determine the effects of the temperature, pH, and duration of the acidic treatment on fucoidan yield, alginate extractability, and the molecular weight of sequentially extracted alginates. Desirability functions were then used to predict the best overall combinations of responses. The most desirable compromise allowed for the recovery of a fucoidan-rich fraction with a yield of 3.75% (w/w of alga) and the sequential extraction of alginates having an average molecular weight of 730. kDa at a yield of 44% (w/w of alga), with low cross-contamination between the products. The optimized acidic treatment could form the basis of an industrial biorefinery process for the production of both fucoidan and alginate.
KW - Biorefinery
KW - Desirability function
KW - Experimental design
KW - Response surface methodology
KW - Sulfated polysaccharides
UR - http://www.scopus.com/inward/record.url?scp=84940765793&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2015.08.103
DO - 10.1016/j.biortech.2015.08.103
M3 - Article
SN - 0960-8524
VL - 197
SP - 302
EP - 309
JO - Bioresource Technology
JF - Bioresource Technology
ER -
