Muscarinic M2-receptors in rat thoracic dorsal root ganglia

Rainer Haberberger, Michael Henrich, Jean Yves Couraud, Wolfgang Kummer

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The occurrence and distribution of the muscarinic M2-receptor subtype (M2R) was investigated in rat thoracic dorsal root ganglia (DRG). Messenger RNA for M2R was demonstrated by RT-PCR in total RNA from DRG. Immunoreactivity to M2R-protein was localized to 26% of sensory neurons, the majority of them (85%) belonging to the size class of 25-40 μm in diameter. Double-labeling (immuno)histochemistry revealed that all M2R-immunoreactive neurons bind the lectin, IB4, whereas they are generally devoid of substance P-immunoreactivity. These data show the presence of M2R on a subpopulation of presumably nociceptive primary afferent neurons, thereby extending previous pharmacological and electrophysiological studies that indicated a role of M2R and/or M4R in inhibition of calcium channel currents in rat sensory neurons (Wanke, E., Bianchi, L., Mantegazza, M., Guatteo, E., Macinelli, E. and Ferroni, A., Muscarinic regulation of Ca2+ currents in rat sensory neurons: channel and receptor types, dose-response relationships and cross-talk pathways.

Original languageEnglish
Pages (from-to)177-180
Number of pages4
JournalNeuroscience letters
Volume266
Issue number3
DOIs
Publication statusPublished - 14 May 1999
Externally publishedYes

Keywords

  • Acetylcholine
  • Cholinergic receptors
  • I-B4
  • Immunohistochemistry
  • Nociceptor
  • Sensory neuron
  • Substance P

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