Muscarinic M2-receptors in rat thoracic dorsal root ganglia

Rainer Haberberger; Michael Henrich; Jean Yves Couraud; Wolfgang Kummer

doi:10.1016/S0304-3940(99)00300-6

Muscarinic M2-receptors in rat thoracic dorsal root ganglia

Rainer Haberberger, Michael Henrich, Jean Yves Couraud, Wolfgang Kummer

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

The occurrence and distribution of the muscarinic M2-receptor subtype (M2R) was investigated in rat thoracic dorsal root ganglia (DRG). Messenger RNA for M2R was demonstrated by RT-PCR in total RNA from DRG. Immunoreactivity to M2R-protein was localized to 26% of sensory neurons, the majority of them (85%) belonging to the size class of 25-40 μm in diameter. Double-labeling (immuno)histochemistry revealed that all M2R-immunoreactive neurons bind the lectin, IB4, whereas they are generally devoid of substance P-immunoreactivity. These data show the presence of M2R on a subpopulation of presumably nociceptive primary afferent neurons, thereby extending previous pharmacological and electrophysiological studies that indicated a role of M2R and/or M4R in inhibition of calcium channel currents in rat sensory neurons (Wanke, E., Bianchi, L., Mantegazza, M., Guatteo, E., Macinelli, E. and Ferroni, A., Muscarinic regulation of Ca²⁺ currents in rat sensory neurons: channel and receptor types, dose-response relationships and cross-talk pathways.

Original language	English
Pages (from-to)	177-180
Number of pages	4
Journal	Neuroscience letters
Volume	266
Issue number	3
DOIs	https://doi.org/10.1016/S0304-3940(99)00300-6
Publication status	Published - 14 May 1999
Externally published	Yes

Keywords

Acetylcholine
Cholinergic receptors
I-B4
Immunohistochemistry
Nociceptor
Sensory neuron
Substance P

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title = "Muscarinic M2-receptors in rat thoracic dorsal root ganglia",

abstract = "The occurrence and distribution of the muscarinic M2-receptor subtype (M2R) was investigated in rat thoracic dorsal root ganglia (DRG). Messenger RNA for M2R was demonstrated by RT-PCR in total RNA from DRG. Immunoreactivity to M2R-protein was localized to 26% of sensory neurons, the majority of them (85%) belonging to the size class of 25-40 μm in diameter. Double-labeling (immuno)histochemistry revealed that all M2R-immunoreactive neurons bind the lectin, IB4, whereas they are generally devoid of substance P-immunoreactivity. These data show the presence of M2R on a subpopulation of presumably nociceptive primary afferent neurons, thereby extending previous pharmacological and electrophysiological studies that indicated a role of M2R and/or M4R in inhibition of calcium channel currents in rat sensory neurons (Wanke, E., Bianchi, L., Mantegazza, M., Guatteo, E., Macinelli, E. and Ferroni, A., Muscarinic regulation of Ca2+ currents in rat sensory neurons: channel and receptor types, dose-response relationships and cross-talk pathways.",

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AU - Henrich, Michael

AU - Couraud, Jean Yves

AU - Kummer, Wolfgang

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AB - The occurrence and distribution of the muscarinic M2-receptor subtype (M2R) was investigated in rat thoracic dorsal root ganglia (DRG). Messenger RNA for M2R was demonstrated by RT-PCR in total RNA from DRG. Immunoreactivity to M2R-protein was localized to 26% of sensory neurons, the majority of them (85%) belonging to the size class of 25-40 μm in diameter. Double-labeling (immuno)histochemistry revealed that all M2R-immunoreactive neurons bind the lectin, IB4, whereas they are generally devoid of substance P-immunoreactivity. These data show the presence of M2R on a subpopulation of presumably nociceptive primary afferent neurons, thereby extending previous pharmacological and electrophysiological studies that indicated a role of M2R and/or M4R in inhibition of calcium channel currents in rat sensory neurons (Wanke, E., Bianchi, L., Mantegazza, M., Guatteo, E., Macinelli, E. and Ferroni, A., Muscarinic regulation of Ca2+ currents in rat sensory neurons: channel and receptor types, dose-response relationships and cross-talk pathways.

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KW - Cholinergic receptors

KW - I-B4

KW - Immunohistochemistry

KW - Nociceptor

KW - Sensory neuron

KW - Substance P

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ER -

Muscarinic M2-receptors in rat thoracic dorsal root ganglia

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Keywords

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