Mutation Rate of Normal and Malignant Human Lymphocytes

Ram Seshadri; Robert J. Kutlaca; Kevin Trainor; Chris Matthews; Alexander A. Morley

Mutation Rate of Normal and Malignant Human Lymphocytes

Ram Seshadri, Robert J. Kutlaca, Kevin Trainor, Chris Matthews, Alexander A. Morley

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Abstract

The genetic stability of normal and neoplastic lymphocytes was compared by using base-line mutation frequency and mutation rate/cell generation. Mutations at the hypoxanthine-guanine phosphoribosyltransferase locus were studied by enumerating thioguanine-resistant cells in a clonogenic assay. The base-line (“spontaneous”) mutation frequency was 1.52 x 10^-6, 6.38 x 10^-6, and 1.06 x 10^-6 for normal cells from three individuals and was 1.16 x 10^-3, 6.08 x 10^-5, and 3.06 x 10^-5 for the three malignant cell lines, Jurkat (JM), HRIK, FMC-Hu1B, respectively. The mutation cell/generation rate was 24.6 x 10^-6, 15 x 10^-8, and 5.5 x 10^-8 for lymphocytes from the three normal individuals, and 666.4 x 10^-8, 52.8 x 10^-8, and 131 x 10^-8 for the three malignant cell lines. The results suggest that neoplastic lymphocytes are more genetically unstable than normal lymphocytes.

Original language	English
Pages (from-to)	407-409
Number of pages	3
Journal	Cancer Research
Volume	47
Issue number	2
Publication status	Published - 15 Jan 1987
Externally published	Yes

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title = "Mutation Rate of Normal and Malignant Human Lymphocytes",

abstract = "The genetic stability of normal and neoplastic lymphocytes was compared by using base-line mutation frequency and mutation rate/cell generation. Mutations at the hypoxanthine-guanine phosphoribosyltransferase locus were studied by enumerating thioguanine-resistant cells in a clonogenic assay. The base-line (“spontaneous”) mutation frequency was 1.52 x 10-6, 6.38 x 10-6, and 1.06 x 10-6 for normal cells from three individuals and was 1.16 x 10-3, 6.08 x 10-5, and 3.06 x 10-5 for the three malignant cell lines, Jurkat (JM), HRIK, FMC-Hu1B, respectively. The mutation cell/generation rate was 24.6 x 10-6, 15 x 10-8, and 5.5 x 10-8 for lymphocytes from the three normal individuals, and 666.4 x 10-8, 52.8 x 10-8, and 131 x 10-8 for the three malignant cell lines. The results suggest that neoplastic lymphocytes are more genetically unstable than normal lymphocytes.",

author = "Ram Seshadri and Kutlaca, {Robert J.} and Kevin Trainor and Chris Matthews and Morley, {Alexander A.}",

year = "1987",

month = jan,

day = "15",

language = "English",

volume = "47",

pages = "407--409",

journal = "Cancer Research",

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TY - JOUR

T1 - Mutation Rate of Normal and Malignant Human Lymphocytes

AU - Seshadri, Ram

AU - Kutlaca, Robert J.

AU - Trainor, Kevin

AU - Matthews, Chris

AU - Morley, Alexander A.

PY - 1987/1/15

Y1 - 1987/1/15

N2 - The genetic stability of normal and neoplastic lymphocytes was compared by using base-line mutation frequency and mutation rate/cell generation. Mutations at the hypoxanthine-guanine phosphoribosyltransferase locus were studied by enumerating thioguanine-resistant cells in a clonogenic assay. The base-line (“spontaneous”) mutation frequency was 1.52 x 10-6, 6.38 x 10-6, and 1.06 x 10-6 for normal cells from three individuals and was 1.16 x 10-3, 6.08 x 10-5, and 3.06 x 10-5 for the three malignant cell lines, Jurkat (JM), HRIK, FMC-Hu1B, respectively. The mutation cell/generation rate was 24.6 x 10-6, 15 x 10-8, and 5.5 x 10-8 for lymphocytes from the three normal individuals, and 666.4 x 10-8, 52.8 x 10-8, and 131 x 10-8 for the three malignant cell lines. The results suggest that neoplastic lymphocytes are more genetically unstable than normal lymphocytes.

AB - The genetic stability of normal and neoplastic lymphocytes was compared by using base-line mutation frequency and mutation rate/cell generation. Mutations at the hypoxanthine-guanine phosphoribosyltransferase locus were studied by enumerating thioguanine-resistant cells in a clonogenic assay. The base-line (“spontaneous”) mutation frequency was 1.52 x 10-6, 6.38 x 10-6, and 1.06 x 10-6 for normal cells from three individuals and was 1.16 x 10-3, 6.08 x 10-5, and 3.06 x 10-5 for the three malignant cell lines, Jurkat (JM), HRIK, FMC-Hu1B, respectively. The mutation cell/generation rate was 24.6 x 10-6, 15 x 10-8, and 5.5 x 10-8 for lymphocytes from the three normal individuals, and 666.4 x 10-8, 52.8 x 10-8, and 131 x 10-8 for the three malignant cell lines. The results suggest that neoplastic lymphocytes are more genetically unstable than normal lymphocytes.

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M3 - Article

C2 - 3466691

AN - SCOPUS:0023135430

SN - 0008-5472

VL - 47

SP - 407

EP - 409

JO - Cancer Research

JF - Cancer Research

IS - 2

ER -

Mutation Rate of Normal and Malignant Human Lymphocytes

Abstract

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