Mutation Rate of Normal and Malignant Human Lymphocytes

Ram Seshadri, Robert J. Kutlaca, Kevin Trainor, Chris Matthews, Alexander A. Morley

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87 Citations (Scopus)

Abstract

The genetic stability of normal and neoplastic lymphocytes was compared by using base-line mutation frequency and mutation rate/cell generation. Mutations at the hypoxanthine-guanine phosphoribosyltransferase locus were studied by enumerating thioguanine-resistant cells in a clonogenic assay. The base-line (“spontaneous”) mutation frequency was 1.52 x 10-6, 6.38 x 10-6, and 1.06 x 10-6 for normal cells from three individuals and was 1.16 x 10-3, 6.08 x 10-5, and 3.06 x 10-5 for the three malignant cell lines, Jurkat (JM), HRIK, FMC-Hu1B, respectively. The mutation cell/generation rate was 24.6 x 10-6, 15 x 10-8, and 5.5 x 10-8 for lymphocytes from the three normal individuals, and 666.4 x 10-8, 52.8 x 10-8, and 131 x 10-8 for the three malignant cell lines. The results suggest that neoplastic lymphocytes are more genetically unstable than normal lymphocytes.

Original languageEnglish
Pages (from-to)407-409
Number of pages3
JournalCancer Research
Volume47
Issue number2
Publication statusPublished - Jan 1987
Externally publishedYes

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    Seshadri, R., Kutlaca, R. J., Trainor, K., Matthews, C., & Morley, A. A. (1987). Mutation Rate of Normal and Malignant Human Lymphocytes. Cancer Research, 47(2), 407-409.