Mutations in human lymphocytes: Effect of X- and UV-irradiation

B. J.S. Sanderson; J. L. Dempsey; A. A. Morley

doi:10.1016/0165-7992(84)90082-4

Mutations in human lymphocytes: Effect of X- and UV-irradiation

B. J.S. Sanderson, J. L. Dempsey, A. A. Morley

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Abstract

The mutagenic effects of X- and UV-irradiation on human lymphocytes were studied using a highly efficient cloning technique. The hypoxanthine-guanine phosphoribosyl-transferase enzyme locus was used to study mutation induction, with mutant cells being selected by their ability to form a clone in the presence of the purine analogue 6-thioguanine. Mutation dose-response curves for X- and UV-irradiation were established by studying lymphocytes from 11 individuals on day 10 after irradiation. The mean mutation frequency of unirradiated lymphocytes was 2.9 × 10^-6 and there were dose-dependent increase to 9.5 × 10^-5 after 400 rad of X-irradiation, and to 5.6 × 10^-5 after 125 erg/mm² of UV. The expression time of X-ray-induced mutations was 3-7 days. Dose-responses were obtained for mutation frequency and survival following X-irradiation of proliferating and non-proliferating lymphocytes from 8 individuals. Compared with non-proliferating lymphocytes, the proliferating lymphocytes developed fewer mutations but had a greater mortality after irradiation.

Original language	English
Pages (from-to)	223-227
Number of pages	5
Journal	Mutation Research Letters
Volume	140
Issue number	4
DOIs	https://doi.org/10.1016/0165-7992(84)90082-4
Publication status	Published - Aug 1984
Externally published	Yes

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title = "Mutations in human lymphocytes: Effect of X- and UV-irradiation",

abstract = "The mutagenic effects of X- and UV-irradiation on human lymphocytes were studied using a highly efficient cloning technique. The hypoxanthine-guanine phosphoribosyl-transferase enzyme locus was used to study mutation induction, with mutant cells being selected by their ability to form a clone in the presence of the purine analogue 6-thioguanine. Mutation dose-response curves for X- and UV-irradiation were established by studying lymphocytes from 11 individuals on day 10 after irradiation. The mean mutation frequency of unirradiated lymphocytes was 2.9 × 10-6 and there were dose-dependent increase to 9.5 × 10-5 after 400 rad of X-irradiation, and to 5.6 × 10-5 after 125 erg/mm2 of UV. The expression time of X-ray-induced mutations was 3-7 days. Dose-responses were obtained for mutation frequency and survival following X-irradiation of proliferating and non-proliferating lymphocytes from 8 individuals. Compared with non-proliferating lymphocytes, the proliferating lymphocytes developed fewer mutations but had a greater mortality after irradiation.",

author = "Sanderson, {B. J.S.} and Dempsey, {J. L.} and Morley, {A. A.}",

year = "1984",

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T2 - Effect of X- and UV-irradiation

AU - Sanderson, B. J.S.

AU - Dempsey, J. L.

AU - Morley, A. A.

PY - 1984/8

Y1 - 1984/8

N2 - The mutagenic effects of X- and UV-irradiation on human lymphocytes were studied using a highly efficient cloning technique. The hypoxanthine-guanine phosphoribosyl-transferase enzyme locus was used to study mutation induction, with mutant cells being selected by their ability to form a clone in the presence of the purine analogue 6-thioguanine. Mutation dose-response curves for X- and UV-irradiation were established by studying lymphocytes from 11 individuals on day 10 after irradiation. The mean mutation frequency of unirradiated lymphocytes was 2.9 × 10-6 and there were dose-dependent increase to 9.5 × 10-5 after 400 rad of X-irradiation, and to 5.6 × 10-5 after 125 erg/mm2 of UV. The expression time of X-ray-induced mutations was 3-7 days. Dose-responses were obtained for mutation frequency and survival following X-irradiation of proliferating and non-proliferating lymphocytes from 8 individuals. Compared with non-proliferating lymphocytes, the proliferating lymphocytes developed fewer mutations but had a greater mortality after irradiation.

AB - The mutagenic effects of X- and UV-irradiation on human lymphocytes were studied using a highly efficient cloning technique. The hypoxanthine-guanine phosphoribosyl-transferase enzyme locus was used to study mutation induction, with mutant cells being selected by their ability to form a clone in the presence of the purine analogue 6-thioguanine. Mutation dose-response curves for X- and UV-irradiation were established by studying lymphocytes from 11 individuals on day 10 after irradiation. The mean mutation frequency of unirradiated lymphocytes was 2.9 × 10-6 and there were dose-dependent increase to 9.5 × 10-5 after 400 rad of X-irradiation, and to 5.6 × 10-5 after 125 erg/mm2 of UV. The expression time of X-ray-induced mutations was 3-7 days. Dose-responses were obtained for mutation frequency and survival following X-irradiation of proliferating and non-proliferating lymphocytes from 8 individuals. Compared with non-proliferating lymphocytes, the proliferating lymphocytes developed fewer mutations but had a greater mortality after irradiation.

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JF - Mutation Research Letters

IS - 4

ER -

Mutations in human lymphocytes: Effect of X- and UV-irradiation

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