Mutations in human lymphocytes studied by an HLA selection system

M. Janatipour, K. J. Trainor, R. Kutlaca, G. Bennett, J. Hay, D. R. Turner, A. A. Morley

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65 Citations (Scopus)

Abstract

Human lymphocytes mutated at the HLA-A2 or HLA-A3 alleles were enumerated and studied by primary selection using antibody and complement, followed by limiting dilution cloning and secondary selection using immunofluorescence or antibody and complement. The geometric mean frequency of in vivo mutant lymphocytes was 3.08×10-5 for the HLA-A2 allele and 4.68×10-6 for the HLA-A3 allele. Mutagenesis by X-radiation or mitomycin produced a dose-related increase in mutant frequency. HLA-B phenotyping and Southern Analysis of the HLA-A gene suggested that mutation was frequently due to gene deletion, which was often substantial.

Original languageEnglish
Pages (from-to)221-226
Number of pages6
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume198
Issue number1
DOIs
Publication statusPublished - Mar 1988
Externally publishedYes

Keywords

  • Autosomal mutation
  • Gene deletion
  • HLA locus
  • Human lymphocyte
  • Human mutagenesis
  • Mutation frequency

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