Mutations in human lymphocytes studied by an HLA selection system

M. Janatipour; K. J. Trainor; R. Kutlaca; G. Bennett; J. Hay; D. R. Turner; A. A. Morley

doi:10.1016/0027-5107(88)90058-9

Mutations in human lymphocytes studied by an HLA selection system

M. Janatipour, K. J. Trainor, R. Kutlaca, G. Bennett, J. Hay, D. R. Turner, A. A. Morley

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Abstract

Human lymphocytes mutated at the HLA-A2 or HLA-A3 alleles were enumerated and studied by primary selection using antibody and complement, followed by limiting dilution cloning and secondary selection using immunofluorescence or antibody and complement. The geometric mean frequency of in vivo mutant lymphocytes was 3.08×10^-5 for the HLA-A2 allele and 4.68×10^-6 for the HLA-A3 allele. Mutagenesis by X-radiation or mitomycin produced a dose-related increase in mutant frequency. HLA-B phenotyping and Southern Analysis of the HLA-A gene suggested that mutation was frequently due to gene deletion, which was often substantial.

Original language	English
Pages (from-to)	221-226
Number of pages	6
Journal	Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume	198
Issue number	1
DOIs	https://doi.org/10.1016/0027-5107(88)90058-9
Publication status	Published - Mar 1988
Externally published	Yes

Keywords

Autosomal mutation
Gene deletion
HLA locus
Human lymphocyte
Human mutagenesis
Mutation frequency

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title = "Mutations in human lymphocytes studied by an HLA selection system",

abstract = "Human lymphocytes mutated at the HLA-A2 or HLA-A3 alleles were enumerated and studied by primary selection using antibody and complement, followed by limiting dilution cloning and secondary selection using immunofluorescence or antibody and complement. The geometric mean frequency of in vivo mutant lymphocytes was 3.08×10-5 for the HLA-A2 allele and 4.68×10-6 for the HLA-A3 allele. Mutagenesis by X-radiation or mitomycin produced a dose-related increase in mutant frequency. HLA-B phenotyping and Southern Analysis of the HLA-A gene suggested that mutation was frequently due to gene deletion, which was often substantial.",

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T1 - Mutations in human lymphocytes studied by an HLA selection system

AU - Janatipour, M.

AU - Trainor, K. J.

AU - Kutlaca, R.

AU - Bennett, G.

AU - Hay, J.

AU - Turner, D. R.

AU - Morley, A. A.

PY - 1988/3

Y1 - 1988/3

N2 - Human lymphocytes mutated at the HLA-A2 or HLA-A3 alleles were enumerated and studied by primary selection using antibody and complement, followed by limiting dilution cloning and secondary selection using immunofluorescence or antibody and complement. The geometric mean frequency of in vivo mutant lymphocytes was 3.08×10-5 for the HLA-A2 allele and 4.68×10-6 for the HLA-A3 allele. Mutagenesis by X-radiation or mitomycin produced a dose-related increase in mutant frequency. HLA-B phenotyping and Southern Analysis of the HLA-A gene suggested that mutation was frequently due to gene deletion, which was often substantial.

AB - Human lymphocytes mutated at the HLA-A2 or HLA-A3 alleles were enumerated and studied by primary selection using antibody and complement, followed by limiting dilution cloning and secondary selection using immunofluorescence or antibody and complement. The geometric mean frequency of in vivo mutant lymphocytes was 3.08×10-5 for the HLA-A2 allele and 4.68×10-6 for the HLA-A3 allele. Mutagenesis by X-radiation or mitomycin produced a dose-related increase in mutant frequency. HLA-B phenotyping and Southern Analysis of the HLA-A gene suggested that mutation was frequently due to gene deletion, which was often substantial.

KW - Autosomal mutation

KW - Gene deletion

KW - HLA locus

KW - Human lymphocyte

KW - Human mutagenesis

KW - Mutation frequency

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Mutations in human lymphocytes studied by an HLA selection system

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