Myb-binding protein 1a (Mybbp1a) regulates levels and processing of pre-ribosomal RNA

J Hochstatter, M Holzel, M Rohrmoser, L Schermelleh, H Leonhardt, Rebecca Keough, Thomas Gonda, Axel Imhof, D Eick, G Langst, A Nemeth

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    22 Citations (Scopus)

    Abstract

    Ribosomal RNA gene transcription, co-transcriptional processing, and ribosome biogenesis are highly coordinated processes that are tightly regulated during cell growth. In this study we discovered that Mybbp1a is associated with both the RNA polymerase I complex and the ribosome biogenesis machinery. Using a reporter assay that uncouples transcription and RNA processing, we show that Mybbp1a represses rRNA gene transcription. In addition, overexpression of the protein reduces RNA polymerase I loading on endogenous rRNA genes as revealed by chromatin immunoprecipitation experiments. Accordingly, depletion of Mybbp1a results in an accumulation of the rRNA precursor in vivo but surprisingly also causes growth arrest of the cells. This effect can be explained by the observation that the modulation of Mybbp1a protein levels results in defects in pre-rRNA processing within the cell. Therefore, the protein may play a dual role in the rRNA metabolism, potentially linking and coordinating ribosomal DNA transcription and pre-rRNA processing to allow for the efficient synthesis of ribosomes.

    Original languageEnglish
    Pages (from-to)24365-24377
    Number of pages13
    JournalJournal of Biological Chemistry
    Volume287
    Issue number29
    DOIs
    Publication statusPublished - 2012

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    Hochstatter, J., Holzel, M., Rohrmoser, M., Schermelleh, L., Leonhardt, H., Keough, R., Gonda, T., Imhof, A., Eick, D., Langst, G., & Nemeth, A. (2012). Myb-binding protein 1a (Mybbp1a) regulates levels and processing of pre-ribosomal RNA. Journal of Biological Chemistry, 287(29), 24365-24377. https://doi.org/10.1074/jbc.M111.303719