Myo-inositol improves growth performance and regulates lipid metabolism of juvenile Chinese mitten crab (Eriocheir sinensis) fed different percentage of lipid

Xianyong Bu, Xiaodan Wang, Zhideng Lin, Chunling Wang, Lingyu Li, Shubin Liu, Qingchao Shi, Jian G. Qin, Liqiao Chen

Research output: Contribution to journalArticlepeer-review

Abstract

This study evaluated the effects of dietary myo-inositol (MI) on growth performance, body composition, antioxidant status and lipid metabolism of juvenile Chinese mitten crab (Eriocheir sinensis) fed different percentage of lipid. Crabs (4.58 ± 0.05 g) were fed four diets including a normal lipid diet (N, containing 7% lipid and 0 mg/kg MI), N with MI supplementation (N+MI, containing 7% lipid and 1600 mg/kg MI), a high lipid diet (H, containing 13% lipid and 0 mg/kg MI) and H with MI supplementation (H+MI, containing 13% lipid and 1600 mg/kg MI) for 8 weeks. The H+MI group showed higher weight gain and specific growth rate than those in the H group. The dietary MI could improve the reduction of whole-body protein content, low MI content in the hepatopancreas, and lipid accumulations in the whole-body, hepatopancreas and muscle as a result of feeding on the high dietary lipid (13%) in crabs. Besides, the crabs fed the H+MI diets increased the activities of antioxidant enzymes, but reduced the malondialdehyde content in hepatopancreas compared with those fed the H diets. Moreover, dietary MI enhanced the expression of genes involved in lipid oxidation and exportation, yet reduced lipid absorption and synthesis genes expression in the hepatopancreas of crabs fed the H diet, which might be related to the activation of IP3R/CaMKKβ/AMPK signalling pathway. This study demonstrates that MI could increase lipid utilization and reduce lipid deposition in the hepatopancreas of E. sinensis fed a high lipid diet through IP3R/CaMKKβ/AMPK activation. This work provides new insights into the function of MI in the diet of crustaceans.

Original languageEnglish
Number of pages13
JournalBritish Journal of Nutrition
Early online date29 Apr 2021
DOIs
Publication statusE-pub ahead of print - 29 Apr 2021

Keywords

  • Eriocheir sinensis
  • High lipid diet
  • Lipid metabolism
  • Myo-inositol
  • Regulatory pathways

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