N-terminal acetylation inhibits protein targeting to the endoplasmic reticulum

Gabriella M A Forte, Martin R Pool, Colin John Stirling

Research output: Contribution to journalArticlepeer-review

138 Citations (Scopus)


Amino-terminal acetylation is probably the most common protein modification in eukaryotes with as many as 50%–80% of proteins reportedly altered in this way. Here we report a systematic analysis of the predicted N-terminal processing of cytosolic proteins versus those destined to be sorted to the secretory pathway. While cytosolic proteins were profoundly biased in favour of processing, we found an equal and opposite bias against such modification for secretory proteins. Mutations in secretory signal sequences that led to their acetylation resulted in mis-sorting to the cytosol in a manner that was dependent upon the N-terminal processing machinery. Hence N-terminal acetylation represents an early determining step in the cellular sorting of nascent polypeptides that appears to be conserved across a wide range of species.
Original languageEnglish
Article numbere1001073
Number of pages13
JournalPLOS Biology
Issue number5
Publication statusPublished - May 2011


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