Nanotopography as a trigger for the microscale, autogenous and passive lysis of erythrocytes

Vy T.H. Pham, Vi Khanh Truong, David E. Mainwaring, Yachong Guo, Vladimir A. Baulin, Mohammad Al Kobaisi, Gediminas Gervinskas, Saulius Juodkazis, Wendy R. Zeng, Pauline P. Doran, Russell J. Crawford, Elena P. Ivanova

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)

Abstract

Microscale devices are increasingly being developed for diagnostic analysis although conventional lysis as an initial step presents limitations due to its scale or complexity. Here, we detail the physical response of erythrocytes to the surface nanoarchitecture of black Si (bSi) and foreshadow their potential in microanalysis. The physical interaction brought about by the spatial convergence of the two topologies: (a) the nanopillar array present on the bSi and (b) the erythrocyte cytoskeleton present on the red blood cells (RBCs), provides spontaneous stress-induced cell deformation, rupture and passive lysis within an elapsed time of ∼3 min from immobilisation to rupture and without external chemical or mechanical intervention. The mechano-responsive bSi surface provides highly active yet autogenous RBC lysis and a prospect as a front-end platform technology in evolving micro-fluidic platforms for cellular analyses.

Original languageEnglish
Pages (from-to)2819-2826
Number of pages8
JournalJOURNAL OF MATERIALS CHEMISTRY B
Volume2
Issue number19
DOIs
Publication statusPublished - 21 May 2014
Externally publishedYes

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