Neurofilament protein triplet immunoreactivity in the dorsal root ganglia of the guinea-pig

James C. Vickers, Marcello Costa

    Research output: Contribution to journalArticlepeer-review

    11 Citations (Scopus)

    Abstract

    Immunoreactivity for the neurofilament protein triplet was investigated in neurons of the dorsal root ganglia of the guinea-pig by using a battery of antibodies. In unfixed tissue, nearly all neurons in these ganglia demonstrated some degree of neurofilament protein triplet immunoreactivity. Large neurons generally displayed intense immunoreactivity, whereas most small to medium-sized neurons showed faint to moderate immunoreactivity. Double-labelling immunofluorescence demonstrated that most antibodies to the individual subunits of the neurofilament protein triplet had the same distribution and intensity of labelling in sensory neurons. Increasing durations of tissue fixation in aldehyde solutions selectively diminished neurofilament protein triplet immunoreactivity in small to medium-sized neurons. Double-labelling with neurofilament protein triplet antibodies in combination with antibodies to other neuronal markers, such as neuron-specific enolase, substance P and tyrosine hydroxylase, showed that tissue processing conditions affect the degree of co-localization of immunoreactivity to the neurofilament protein triplet and to these other neuronal markers. These results indicate that, with a judicious manipulation of the duration of tissue fixation, neurofilament protein triplet immunoreactivity can be used in combination with other neuronal markers to distinguish groups of neurons according to their size and chemical coding.

    Original languageEnglish
    Pages (from-to)159-167
    Number of pages9
    JournalCell and Tissue Research
    Volume265
    Issue number1
    DOIs
    Publication statusPublished - Jul 1991

    Keywords

    • Dorsal root ganglia
    • Guinea-pig
    • Neurofilament protein
    • Neuron-specific enolase
    • Substance P
    • Tyrosine hydroxylase

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