Neuronal medium that supports basic synaptic functions and activity of human neurons in vitro

Cedric Bardy, Mark van den Hurk, Tameji Eames, Cynthia Marchand, Ruben Hernandez, Mariko Kellogg, Mark Gorris, Ben Galet, Vanessa Palomares, Joshua Brown, Anne Bang, Jerome Mertens, Lena Bohnke, Leah Boyer, Suzanne Simon, Fred Gage

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    127 Citations (Scopus)

    Abstract

    Human cell reprogramming technologies offer access to live human neurons from patients and provide a new alternative for modeling neurological disorders in vitro. Neural electrical activity is the essence of nervous system function in vivo. Therefore, we examined neuronal activity in media widely used to culture neurons. We found that classic basal media, as well as serum, impair action potential generation and synaptic communication. To overcome this problem, we designed a new neuronal medium (BrainPhys basal + serum-free supplements) in which we adjusted the concentrations of inorganic salts, neuroactive amino acids, and energetic substrates. We then tested that this medium adequately supports neuronal activity and survival of human neurons in culture. Long-term exposure to this physiological medium also improved the proportion of neurons that were synaptically active. The medium was designed to culture human neurons but also proved adequate for rodent neurons. The improvement in BrainPhys basal medium to support neurophysiological activity is an important step toward reducing the gap between brain physiological conditions in vivo and neuronal models in vitro.

    Original languageEnglish
    Pages (from-to)E2725-E2734
    Number of pages10
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume112
    Issue number20
    DOIs
    Publication statusPublished - 2015

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  • Cite this

    Bardy, C., van den Hurk, M., Eames, T., Marchand, C., Hernandez, R., Kellogg, M., Gorris, M., Galet, B., Palomares, V., Brown, J., Bang, A., Mertens, J., Bohnke, L., Boyer, L., Simon, S., & Gage, F. (2015). Neuronal medium that supports basic synaptic functions and activity of human neurons in vitro. Proceedings of the National Academy of Sciences of the United States of America, 112(20), E2725-E2734. https://doi.org/10.1073/pnas.1504393112