NEUTROPHIL-MEDIATED ENDOTHELIAL INJURY IN HAEMOLYTIC URAEMIC SYNDROME

Kevin D. Forsyth, Margaret M. Fitzpatrick, Anna C. Simpson, T. Martin Barratt, Roland J. Levinsky

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Abstract

Neutrophil leucocytosis is associated with a poor outcome in the haemolytic uraemic syndrome (HUS). This study tested the hypothesis that neutrophils from HUS patients are activated and through release of their intracellular contents damage endothelium. The proportion of neutrophils adhering to endothelium in culture was twice as high for HUS patients' neutrophils as for control neutrophils (n=12). In addition, these neutrophils induced endothelial injury, assessed morphologically by degradation of endothelial cell fibronectin. In an attempt to inhibit neutrophil adhesion and subsequent endothelial damage the hyperadhesive neutrophils from HUS patients were incubated with a CD18 antibody directed against the common β chain of the leucocyte integrin molecules. The CD18 antibody was able to abrogate endothelial damage in four of the ten subjects studied. These observations suggest that the neutrophil is of prime pathophysiological importance in HUS, and that methods aimed at reducing neutrophil adhesion and neutrophil-mediated endothelial damage are likely to be beneficial.

Original languageEnglish
Pages (from-to)411-414
Number of pages4
JournalThe Lancet
Volume334
Issue number8660
DOIs
Publication statusPublished - 19 Aug 1989
Externally publishedYes

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    Forsyth, K. D., Fitzpatrick, M. M., Simpson, A. C., Barratt, T. M., & Levinsky, R. J. (1989). NEUTROPHIL-MEDIATED ENDOTHELIAL INJURY IN HAEMOLYTIC URAEMIC SYNDROME. The Lancet, 334(8660), 411-414. https://doi.org/10.1016/S0140-6736(89)90591-6