New insights into the infection process of Rhynchosporium secalis in barley using GFP

Katherine Linsell, Felicity Keiper, Angus Forgan, Klaus Oldach

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    13 Citations (Scopus)


    Through the use of a Rhynchosporium secalis isolate transformed with the green fluorescent protein gene and LASER scanning confocal microscopy (LSCM), fungal development during the R. secalis/barley interaction was analysed. Each infection stage was investigated from 0.5. h to 14. days post-inoculation (p.i.) with extensive sampling within the first 48. h p.i. Early germination events were observed that had not been previously described. A specific time of germination was noted, with germ tube formation appearing as early as 1. h p.i. Conidia were observed within anticlinal grooves of epidermal cells and the formation of bubbles within these pectin-rich regions was observed within 24. h p.i. The study reports R. secalis pectinase production and suggests degradation of these pectin-rich regions. Reactive oxygen species were present during early penetration, 3. h p.i. and co-localised with fungal development. LSCM allowed the visualisation of fungal growth deep within tissues at the later stage of the infection.

    Original languageEnglish
    Pages (from-to)124-131
    Number of pages8
    JournalFungal Genetics and Biology
    Issue number2
    Publication statusPublished - Feb 2011


    • Barley
    • GFP
    • Infection
    • LASER scanning confocal microscopy (LSCM)
    • Pectinase
    • Rhynchosporium secalis
    • ROS
    • Scald


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