Norbornene Probes for the Detection of Cysteine Sulfenic Acid in Cells

Lisa J. Alcock, Bruno L. Oliveira, Michael J. Deery, Tara L. Pukala, Michael V. Perkins, Gonçalo J. L. Bernardes, Justin M. Chalker

Research output: Contribution to journalLetterpeer-review

23 Citations (Scopus)

Abstract

Norbornene derivatives were validated as probes for cysteine sulfenic acid on proteins and in live cells. Trapping sulfenic acids with norbornene probes is highly selective and revealed a different reactivity profile than the traditional dimedone reagent. The norbornene probe also revealed a superior chemoselectivity when compared to a commonly used dimedone probe. Together, these results advance the study of cysteine oxidation in biological systems.

Original languageEnglish
Pages (from-to)594-598
Number of pages5
JournalACS Chemical Biology
Volume14
Issue number4
Early online date2019
DOIs
Publication statusPublished - 19 Apr 2019

Keywords

  • Norbornene Probes
  • Cysteine Sulfenic Acid
  • proteins
  • cells
  • biological systems

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