Norbornene Probes for the Detection of Cysteine Sulfenic Acid in Cells

Lisa J. Alcock; Bruno L. Oliveira; Michael J. Deery; Tara L. Pukala; Michael V. Perkins; Gonçalo J. L. Bernardes; Justin M. Chalker

doi:10.1021/acschembio.8b01104

Norbornene Probes for the Detection of Cysteine Sulfenic Acid in Cells

Lisa J. Alcock, Bruno L. Oliveira, Michael J. Deery, Tara L. Pukala, Michael V. Perkins, Gonçalo J. L. Bernardes, Justin M. Chalker

Research output: Contribution to journal › Letter › peer-review

37 Citations (Scopus)

Abstract

Norbornene derivatives were validated as probes for cysteine sulfenic acid on proteins and in live cells. Trapping sulfenic acids with norbornene probes is highly selective and revealed a different reactivity profile than the traditional dimedone reagent. The norbornene probe also revealed a superior chemoselectivity when compared to a commonly used dimedone probe. Together, these results advance the study of cysteine oxidation in biological systems.

Original language	English
Pages (from-to)	594-598
Number of pages	5
Journal	ACS Chemical Biology
Volume	14
Issue number	4
Early online date	2019
DOIs	https://doi.org/10.1021/acschembio.8b01104
Publication status	Published - 19 Apr 2019

Keywords

Norbornene Probes
Cysteine Sulfenic Acid
proteins
cells
biological systems

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10.1021/acschembio.8b01104Licence: In Copyright

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title = "Norbornene Probes for the Detection of Cysteine Sulfenic Acid in Cells",

abstract = "Norbornene derivatives were validated as probes for cysteine sulfenic acid on proteins and in live cells. Trapping sulfenic acids with norbornene probes is highly selective and revealed a different reactivity profile than the traditional dimedone reagent. The norbornene probe also revealed a superior chemoselectivity when compared to a commonly used dimedone probe. Together, these results advance the study of cysteine oxidation in biological systems.",

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author = "Alcock, {Lisa J.} and Oliveira, {Bruno L.} and Deery, {Michael J.} and Pukala, {Tara L.} and Perkins, {Michael V.} and Bernardes, {Gon{\c c}alo J. L.} and Chalker, {Justin M.}",

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doi = "10.1021/acschembio.8b01104",

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T1 - Norbornene Probes for the Detection of Cysteine Sulfenic Acid in Cells

AU - Alcock, Lisa J.

AU - Oliveira, Bruno L.

AU - Deery, Michael J.

AU - Pukala, Tara L.

AU - Perkins, Michael V.

AU - Bernardes, Gonçalo J. L.

AU - Chalker, Justin M.

PY - 2019/4/19

Y1 - 2019/4/19

N2 - Norbornene derivatives were validated as probes for cysteine sulfenic acid on proteins and in live cells. Trapping sulfenic acids with norbornene probes is highly selective and revealed a different reactivity profile than the traditional dimedone reagent. The norbornene probe also revealed a superior chemoselectivity when compared to a commonly used dimedone probe. Together, these results advance the study of cysteine oxidation in biological systems.

AB - Norbornene derivatives were validated as probes for cysteine sulfenic acid on proteins and in live cells. Trapping sulfenic acids with norbornene probes is highly selective and revealed a different reactivity profile than the traditional dimedone reagent. The norbornene probe also revealed a superior chemoselectivity when compared to a commonly used dimedone probe. Together, these results advance the study of cysteine oxidation in biological systems.

KW - Norbornene Probes

KW - Cysteine Sulfenic Acid

KW - proteins

KW - cells

KW - biological systems

UR - http://www.scopus.com/inward/record.url?scp=85064812432&partnerID=8YFLogxK

UR - http://purl.org/au-research/grants/ARC/DE150101863

U2 - 10.1021/acschembio.8b01104

DO - 10.1021/acschembio.8b01104

M3 - Letter

SN - 1554-8929

VL - 14

SP - 594

EP - 598

JO - ACS Chemical Biology

JF - ACS Chemical Biology

IS - 4

ER -

Norbornene Probes for the Detection of Cysteine Sulfenic Acid in Cells

Abstract

Keywords

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