Novel Sensitive Real-Time PCR for Quantiﬁcation of Bacterial 16SrRNA Genes in Plasma of HIV-Infected Patients as a Marker forMicrobial Translocation

M. Kramski; A. J. Gaeguta; G. F. Lichtfuss; R. Rajasuriar; S. M. Crowe; M. A. French; S. R. Lewin; R. J. Center; D. F.J. Purcell

doi:10.1128/JCM.01018-11

Novel Sensitive Real-Time PCR for Quantiﬁcation of Bacterial 16SrRNA Genes in Plasma of HIV-Infected Patients as a Marker forMicrobial Translocation

M. Kramski
, A. J. Gaeguta
, G. F. Lichtfuss
, R. Rajasuriar
, S. M. Crowe
, M. A. French
, S. R. Lewin
, R. J. Center
, D. F.J. Purcell

Research output: Contribution to journal › Article › peer-review

48 Citations (Scopus)

Abstract

We developed a real-time PCR to quantify 16S rRNA gene levels in plasma from HIV-infected patients as a marker of microbial translocation. The assay uses shrimp nuclease (SNuc) to eliminate DNA contamination, giving high sensitivity and low variability. The 16S rRNA gene levels measured in plasma from HIV patients correlated significantly with lipopolysaccharide levels.

Original language	English
Pages (from-to)	3691-3693
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	49
Issue number	10
DOIs	https://doi.org/10.1128/JCM.01018-11
Publication status	Published - Oct 2011
Externally published	Yes

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Kramski, M., Gaeguta, A. J., Lichtfuss, G. F., Rajasuriar, R., Crowe, S. M., French, M. A., Lewin, S. R., Center, R. J., & Purcell, D. F. J. (2011). Novel Sensitive Real-Time PCR for Quantiﬁcation of Bacterial 16SrRNA Genes in Plasma of HIV-Infected Patients as a Marker forMicrobial Translocation. Journal of Clinical Microbiology, 49(10), 3691-3693. https://doi.org/10.1128/JCM.01018-11

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abstract = "We developed a real-time PCR to quantify 16S rRNA gene levels in plasma from HIV-infected patients as a marker of microbial translocation. The assay uses shrimp nuclease (SNuc) to eliminate DNA contamination, giving high sensitivity and low variability. The 16S rRNA gene levels measured in plasma from HIV patients correlated significantly with lipopolysaccharide levels.",

author = "M. Kramski and Gaeguta, \{A. J.\} and Lichtfuss, \{G. F.\} and R. Rajasuriar and Crowe, \{S. M.\} and French, \{M. A.\} and Lewin, \{S. R.\} and Center, \{R. J.\} and Purcell, \{D. F.J.\}",

year = "2011",

month = oct,

doi = "10.1128/JCM.01018-11",

language = "English",

volume = "49",

pages = "3691--3693",

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Kramski, M, Gaeguta, AJ, Lichtfuss, GF, Rajasuriar, R, Crowe, SM, French, MA, Lewin, SR, Center, RJ & Purcell, DFJ 2011, 'Novel Sensitive Real-Time PCR for Quantiﬁcation of Bacterial 16SrRNA Genes in Plasma of HIV-Infected Patients as a Marker forMicrobial Translocation', Journal of Clinical Microbiology, vol. 49, no. 10, pp. 3691-3693. https://doi.org/10.1128/JCM.01018-11

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AU - Gaeguta, A. J.

AU - Lichtfuss, G. F.

AU - Rajasuriar, R.

AU - Crowe, S. M.

AU - French, M. A.

AU - Lewin, S. R.

AU - Center, R. J.

AU - Purcell, D. F.J.

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AB - We developed a real-time PCR to quantify 16S rRNA gene levels in plasma from HIV-infected patients as a marker of microbial translocation. The assay uses shrimp nuclease (SNuc) to eliminate DNA contamination, giving high sensitivity and low variability. The 16S rRNA gene levels measured in plasma from HIV patients correlated significantly with lipopolysaccharide levels.

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DO - 10.1128/JCM.01018-11

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AN - SCOPUS:80053474363

SN - 0095-1137

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IS - 10

ER -

Novel Sensitive Real-Time PCR for Quantiﬁcation of Bacterial 16SrRNA Genes in Plasma of HIV-Infected Patients as a Marker forMicrobial Translocation

Abstract

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