One-step isolation of plant DNA suitable for PCR amplification

Karen Burr, Ross Harper, Adrian Linacre

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

We report a one-step extraction technique for the isolation of plant DNA. DNA suitable for amplification by PCR can be produced from leaf material smaller than 0.3 mm2 in less than 20 min, with no tube changes. The method was tested on several plant species. The described method was found to extract DNA that could be amplified without any further purification or treatment. The isolated DNA was amplified using a universal chloroplast primer set. The method was validated by comparing size of PCR products generated by the novel method to PCR products generated using standard DNA isolation techniques.

Original languageEnglish
Pages (from-to)367-371
Number of pages5
JournalPlant Molecular Biology Reporter
Volume19
Issue number4
DOIs
Publication statusPublished - Dec 2001
Externally publishedYes

Keywords

  • Chloroplast markers
  • DNA isolation
  • Grasses
  • RAPD

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