One-step isolation of plant DNA suitable for PCR amplification

Karen Burr; Ross Harper; Adrian Linacre

doi:10.1007/BF02772835

One-step isolation of plant DNA suitable for PCR amplification

Karen Burr, Ross Harper, Adrian Linacre

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

We report a one-step extraction technique for the isolation of plant DNA. DNA suitable for amplification by PCR can be produced from leaf material smaller than 0.3 mm² in less than 20 min, with no tube changes. The method was tested on several plant species. The described method was found to extract DNA that could be amplified without any further purification or treatment. The isolated DNA was amplified using a universal chloroplast primer set. The method was validated by comparing size of PCR products generated by the novel method to PCR products generated using standard DNA isolation techniques.

Original language	English
Pages (from-to)	367-371
Number of pages	5
Journal	Plant Molecular Biology Reporter
Volume	19
Issue number	4
DOIs	https://doi.org/10.1007/BF02772835
Publication status	Published - Dec 2001
Externally published	Yes

Keywords

Chloroplast markers
DNA isolation
Grasses
RAPD

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title = "One-step isolation of plant DNA suitable for PCR amplification",

abstract = "We report a one-step extraction technique for the isolation of plant DNA. DNA suitable for amplification by PCR can be produced from leaf material smaller than 0.3 mm2 in less than 20 min, with no tube changes. The method was tested on several plant species. The described method was found to extract DNA that could be amplified without any further purification or treatment. The isolated DNA was amplified using a universal chloroplast primer set. The method was validated by comparing size of PCR products generated by the novel method to PCR products generated using standard DNA isolation techniques.",

keywords = "Chloroplast markers, DNA isolation, Grasses, RAPD",

author = "Karen Burr and Ross Harper and Adrian Linacre",

year = "2001",

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AU - Harper, Ross

AU - Linacre, Adrian

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AB - We report a one-step extraction technique for the isolation of plant DNA. DNA suitable for amplification by PCR can be produced from leaf material smaller than 0.3 mm2 in less than 20 min, with no tube changes. The method was tested on several plant species. The described method was found to extract DNA that could be amplified without any further purification or treatment. The isolated DNA was amplified using a universal chloroplast primer set. The method was validated by comparing size of PCR products generated by the novel method to PCR products generated using standard DNA isolation techniques.

KW - Chloroplast markers

KW - DNA isolation

KW - Grasses

KW - RAPD

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DO - 10.1007/BF02772835

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JO - Plant Molecular Biology Reporter

JF - Plant Molecular Biology Reporter

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ER -

One-step isolation of plant DNA suitable for PCR amplification

Abstract

Keywords

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