@inbook{deeefe05c08844a2960810c8dc949471,
title = "Optical Control of Ligand-Gated Ion Channels",
abstract = "In the vibrant field of optogenetics, optics and genetic targeting are combined to commandeer cellular functions, such as the neuronal action potential, by optically stimulating light-sensitive ion channels expressed in the cell membrane. One broadly applicable manifestation of this approach are covalently attached photochromic tethered ligands (PTLs) that allow activating ligand-gated ion channels with outstanding spatial and temporal resolution. Here, we describe all steps towards the successful development and application of PTL-gated ion channels in cell lines and primary cells. The basis for these experiments forms a combination of molecular modeling, genetic engineering, cell culture, and electrophysiology. The light-gated glutamate receptor (LiGluR), which consists of the PTL-functionalized GluK2 receptor, serves as a model.",
keywords = "Azobenzene, Glutamate receptor, LiGluR, Optical switch, Optochemical genetics, Optogenetics, Photochromic tethered ligand",
author = "Stephanie Szobota and Catherine McKenzie and Harald Janovjak",
year = "2013",
doi = "10.1007/978-1-62703-351-0_32",
language = "English",
isbn = "9781627033503",
series = "Methods in Molecular Biology",
pages = "417--435",
editor = "Nikita Gamper",
booktitle = "Ion Channels",
}