Uniform design method was applied for culture medium optimization of a marine microalga, Platymonas subcordiformis. Using the uniform design table U10*(108), three key medium ingredients (nitrate-N, phosphate-P, and Tris-HAc buffer) were chosen as optimizing factors. Their effects on the pH variation, photochemical activity of photosystem II (PSII) and light harvesting pigment of microalga cultures were examined. The optimum medium ingredients were obtained as follows: NO3--N 9.5 mmol/L, PO43--P 0.34 mmol/L, Tris-HAc buffer 6.7 mmol/L, under which P. subcordiformis cells could grow efficiently, reaching a density of (8.28±0.51) × l06 mL-1 within 15 d. It was found that the buffer Tris-HAc maintained a stable pH value, and high NO3--N level improved the photosynthetic efficiency and growth rate of cells.
|Number of pages||5|
|Journal||Guocheng Gongcheng Xuebao/The Chinese Journal of Process Engineering|
|Publication status||Published - 1 Dec 2007|
- Culture medium
- Photosynthetic efficiency
- Platymonas subcordiformis
- Uniform design