Optimization of culture medium and photosynthetic characteristics of platymonas subcordiformis

Yuan Liu, Zhao An Chen, Hong Bin Lu, Chang Fa Liu, Mei Fang Jin, Zhen Guo, Wei Zhang

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Uniform design method was applied for culture medium optimization of a marine microalga, Platymonas subcordiformis. Using the uniform design table U10*(108), three key medium ingredients (nitrate-N, phosphate-P, and Tris-HAc buffer) were chosen as optimizing factors. Their effects on the pH variation, photochemical activity of photosystem II (PSII) and light harvesting pigment of microalga cultures were examined. The optimum medium ingredients were obtained as follows: NO3--N 9.5 mmol/L, PO43--P 0.34 mmol/L, Tris-HAc buffer 6.7 mmol/L, under which P. subcordiformis cells could grow efficiently, reaching a density of (8.28±0.51) × l06 mL-1 within 15 d. It was found that the buffer Tris-HAc maintained a stable pH value, and high NO3--N level improved the photosynthetic efficiency and growth rate of cells.

Original languageEnglish
Pages (from-to)1197-1201
Number of pages5
JournalGuocheng Gongcheng Xuebao/The Chinese Journal of Process Engineering
Volume7
Issue number6
Publication statusPublished - 1 Dec 2007
Externally publishedYes

Keywords

  • Culture medium
  • Photosynthetic efficiency
  • Platymonas subcordiformis
  • Uniform design

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