Optimization of flow cytometry methods to differentiate epithelial cell origin

Ishana Somers, Amy Britton, Giles Best, Duncan Taylor, Roland van Oorschot, Mariya Goray

Research output: Contribution to journalMeeting Abstractpeer-review

Abstract

The ability to link a DNA profile to a source of biological material has significant implications in forensic investigations. Epithelial cells are the main constituent of many of the samples collected as part of forensic casework. However, it is currently not possible to distinguish between or isolate epithelial cells from different anatomical regions. Flow cytometry represents a potential method that could be used in this context. This study was conducted to optimize procedures for the collection and processing of epidermal, buccal, vaginal and penile epithelial cells prior to analysis by flow cytometry.
Original languageEnglish
Pages (from-to)135-138
Number of pages4
JournalAustralian Journal of Forensic Sciences
Volume56
Issue numberSupplement 1
Early online date28 Apr 2024
DOIs
Publication statusPublished - 2024
Event23rd Triennial Meeting of the International Association of Forensic Science: in conjunction with the 26th Symposium of the Australian & New Zealand Forensic Science Society - Sydney International Convention Centre, Sydney, Australia
Duration: 20 Nov 202324 Nov 2023
Conference number: 23
https://iafs2023.com.au/

Keywords

  • Fluorescence-activated cell sorting
  • imaging flow cytometry
  • epithelial cell
  • forensics

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