Combined fluorescence serotonin immunohistochemistry and retrograde transport labelling with Fast blue and Fluoro-gold were used to identify serotonin-immunoreactive neurons in the midbrain and pons which project to the region of the arcuate and ventromedial hypothalamic nuclei. Approximately 90% of doubly labelled neurons were located in the 3 major mesencephalic serotonin-containing cell groups: dorsal raphe (38%), median raphe (21%) and medial lemniscus group (29%). Within these groups, there were numerous non-retrogradely labelled serotonin-immunoreactive neurons as well as numerous non-serotonin-immunoreactive retrogradely labelled neurons. No doubly neurons were observed caudal to raphe pontis non-serotnin immunoreactive neurons were retrogradely labelled in the more caudal raphe nuclei.
- Fast blue
- Medial basal hypothalamus