Abstract
Combined fluorescence serotonin immunohistochemistry and retrograde transport labelling with Fast blue and Fluoro-gold were used to identify serotonin-immunoreactive neurons in the midbrain and pons which project to the region of the arcuate and ventromedial hypothalamic nuclei. Approximately 90% of doubly labelled neurons were located in the 3 major mesencephalic serotonin-containing cell groups: dorsal raphe (38%), median raphe (21%) and medial lemniscus group (29%). Within these groups, there were numerous non-retrogradely labelled serotonin-immunoreactive neurons as well as numerous non-serotonin-immunoreactive retrogradely labelled neurons. No doubly neurons were observed caudal to raphe pontis non-serotnin immunoreactive neurons were retrogradely labelled in the more caudal raphe nuclei.
Original language | English |
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Pages (from-to) | 170-173 |
Number of pages | 4 |
Journal | Brain Research |
Volume | 418 |
Issue number | 1 |
DOIs | |
Publication status | Published - 18 Aug 1987 |
Keywords
- Fast blue
- Fluoro-gold
- Immunohistochemistry
- Medial basal hypothalamus
- Midbrain
- Pons
- Serotonin