TY - JOUR
T1 - Oxidative stress induced by plasma-activated water stimulates astaxanthin production in Phaffia rhodozyma
AU - Li, Wenshao
AU - Luna-Flores, Carlos H.
AU - Anangi, Raveendra
AU - Zhou, Renwu
AU - Tan, Xinle
AU - Jessen, Marius
AU - Liu, Lian
AU - Zhou, Rusen
AU - Zhang, Tianqi
AU - Gissibl, Alexander
AU - Cullen, Patrick J.
AU - Ostrikov, Kostya (Ken)
AU - Speight, Robert E.
PY - 2023/2
Y1 - 2023/2
N2 - Astaxanthin is used extensively in the nutraceutical, aquaculture, and cosmetic industries. The current market necessitates higher astaxanthin production from Phaffia rhodozyma (P. rhodozyma) due to its higher cost compared to chemical synthesis. In this study, a bubble discharge reactor was developed to generate plasma-activated water (PAW) to produce PAW-made yeast malt (YM) medium. Due to oxidative stress induced by PAW, strains cultured in 15 and 30 min-treated PAW-made medium produced 7.9 ± 1.2 % and 12.6 ± 1.4 % more carotenoids with 15.5 ± 3.3 % and 22.1 ± 1.3 % more astaxanthin, respectively. Reactive oxygen species (ROS) assay results showed that ROS generated by plasma-water interactions elevated intracellular ROS levels. Proteomic analysis revealed increased expression of proteins involved in the cellular response to oxidative stress as well as carotenoid biosynthesis, both of which contribute to higher yields of astaxanthin. Overall, this study supports the potential of PAW to increase astaxanthin yields for industrial-scale production.
AB - Astaxanthin is used extensively in the nutraceutical, aquaculture, and cosmetic industries. The current market necessitates higher astaxanthin production from Phaffia rhodozyma (P. rhodozyma) due to its higher cost compared to chemical synthesis. In this study, a bubble discharge reactor was developed to generate plasma-activated water (PAW) to produce PAW-made yeast malt (YM) medium. Due to oxidative stress induced by PAW, strains cultured in 15 and 30 min-treated PAW-made medium produced 7.9 ± 1.2 % and 12.6 ± 1.4 % more carotenoids with 15.5 ± 3.3 % and 22.1 ± 1.3 % more astaxanthin, respectively. Reactive oxygen species (ROS) assay results showed that ROS generated by plasma-water interactions elevated intracellular ROS levels. Proteomic analysis revealed increased expression of proteins involved in the cellular response to oxidative stress as well as carotenoid biosynthesis, both of which contribute to higher yields of astaxanthin. Overall, this study supports the potential of PAW to increase astaxanthin yields for industrial-scale production.
KW - Astaxanthin
KW - Oxidative stress
KW - Phaffia rhodozyma
KW - Plasma-activated water
KW - Reactive oxygen species
UR - http://www.scopus.com/inward/record.url?scp=85142668837&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2022.128370
DO - 10.1016/j.biortech.2022.128370
M3 - Article
C2 - 36423765
AN - SCOPUS:85142668837
SN - 0960-8524
VL - 369
JO - Bioresource Technology
JF - Bioresource Technology
M1 - 128370
ER -
