Paralogous genes involved in juvenile hormone action in Drosophila melanogaster

Aaron Baumann, Joshua Barry, Shaoli Wang, Y Fujiwara, Thomas Wilson

    Research output: Contribution to journalArticle

    47 Citations (Scopus)

    Abstract

    Juvenile hormone (JH) is critical for multiple aspects of insect development and physiology. Although roles for the hormone have received considerable study, an understanding of the molecules necessary for JH action in insects has been frustratingly slow to evolve. Methoprene-tolerant (Met) in Drosophila melanogaster fulfills many of the requirements for a hormone receptor gene. A paralogous gene, germ-cell expressed (gce), possesses homology and is a candidate as a Met partner in JH action. Expression of gce was found to occur at multiple times and in multiple tissues during development, similar to that previously found for Met. To probe roles of this gene in JH action, we carried out in vivo gce over- and underexpression studies. We show by overexpression studies that gce can substitute in vivo for Met, alleviating preadult but not adult phenotypic characters. We also demonstrate that RNA interference-driven knockdown of gce expression in transgenic flies results in preadult lethality in the absence of MET. These results show that (1) unlike Met, gce is a vital gene and shows functional flexibility and (2) both gene products appear to promote JH action in preadult but not adult development.

    Original languageEnglish
    Pages (from-to)1327-1336
    Number of pages10
    JournalGenetics
    Volume185
    Issue number4
    DOIs
    Publication statusPublished - 2010

    Fingerprint Dive into the research topics of 'Paralogous genes involved in juvenile hormone action in Drosophila melanogaster'. Together they form a unique fingerprint.

  • Cite this

    Baumann, A., Barry, J., Wang, S., Fujiwara, Y., & Wilson, T. (2010). Paralogous genes involved in juvenile hormone action in Drosophila melanogaster. Genetics, 185(4), 1327-1336. https://doi.org/10.1534/genetics.110.116962