Pathway-specific expression of PKC and PKA in sympathetic neurons

Ian L. Gibbins, Sue E. Matthew, Phillip Jobling

    Research output: Contribution to journalArticlepeer-review

    11 Citations (Scopus)


    We used multiple-labelling immunofluorescence, intracellular dye injection, electrophysiological recording and confocal microscopy to examine the expression of immunoreactivity to protein kinase C (PKC) and protein kinase A (PKA) in sympathetic ganglia of guinea-pigs. PKCα and PKCγ were widespread in vasoconstrictor and pilomotor neurons. High levels of PKA RIIα and RIIβ were restricted to neurons that lacked significant expression of PKC, including somatostatin-containing neurons projecting to the gut, and non-noradrenergic vasodilator neurons. In coeliac ganglia, most neurons with PKC contained neuropeptide Y and displayed phasic patterns of action potential firing, often with a long after-hyperpolarization. Tonically firing neurons lacked both neuropeptide Y and PKC. These results show remarkably pathway-specific expression of protein kinases in functionally identified populations of sympathetic neurons.

    Original languageEnglish
    Pages (from-to)975-979
    Number of pages5
    Issue number5
    Publication statusPublished - 6 Apr 1999


    • Coeliac ganglion
    • Electrophysiology
    • Guinea- pig
    • Immunohistochemistry
    • Isoforms
    • Neuropeptide Y
    • Neuropeptides
    • Protein kinase
    • Somatostatin
    • Vasoactive intestinal peptide


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