Performance of a method for weighting a range in the number of contributors in probabilistic genotyping

Catherine McGovern, Kevin Cheng, Hannah Kelly, Anne Ciecko, Duncan Taylor, John S. Buckleton, Jo Anne Bright

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Uncertainty in the assignment of the number of contributors (NoC) can be encountered, particularly in higher-order mixtures, where alleles may be shared between contributors, may have dropped out, or may be masked by the stutter artefacts or allelic peaks of a more dominant contributor. Most probabilistic genotyping software requires the assignment of NoC prior to interpretation. NoC has been described as a nuisance parameter. Taylor et al. [1] describe a method to weigh the probability of the profile under different values of N and incorporate this into a likelihood ratio (LR). Within this paper we explore the performance of this variable number of contributors (varNoC) method programmed within the probabilistic genotyping software STRmix™. The desired combination of performance and runtime was obtained using the default STRmix™ version 2.7 MCMC settings in conjunction with a 2.5 % hyper-rectangle range, at least 10,000 naïve MC iterations and 8 MCMC chains. The varNoC LR demonstrated the typical sensitivity and specificity behaviour seen in previous studies, with a high level of reproducibility given repeat analyses. Profiles previously demonstrating ambiguity in the NoC assigned using conventional estimation methods, were able to be reliably interpreted and a varNoC LR assigned.

Original languageEnglish
Article number102352
Number of pages9
JournalForensic Science International: Genetics
Volume48
DOIs
Publication statusPublished - Sep 2020

Keywords

  • Forensic DNA
  • Number of contributors
  • Probabilistic genotyping
  • STRmix

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