Phentolamine increases neuronal binding and retrograde transport of dopamine β-hydroxylase antibodies

G. J. Lees, L. B. Geffen, R. A. Rush

    Research output: Contribution to journalArticle

    11 Citations (Scopus)

    Abstract

    When 125I-labelled antibodies against dopamine β-hydroxylase (DBH) were injected into the anterior eye chamber of guinea-pigs they bound to sympathetic nerve terminals, were internalized into the axons and retrogradely transported to the ipsilateral superior cervical ganglion (SCG). This process was demonstrated to depend on specific binding sites since neutralized antibodies were not taken up and transported. The α-receptor antagonist phentolamine caused a 2.5-fold increase in binding in the iris and a 2.1-fold increase in accumulation of [125I]anti-DBH in the SCG. The results demonstrate that retrograde axonal transport of synaptic vesicle components is coupled to their turnover in nerve terminals.

    Original languageEnglish
    Pages (from-to)115-118
    Number of pages4
    JournalNeuroscience letters
    Volume22
    Issue number2
    DOIs
    Publication statusPublished - 10 Mar 1981

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