Phosphatidylinositol(4,5)bisphosphate coordinates actin-mediated mobilization and translocation of secretory vesicles to the plasma membrane of chromaffin cells

Peter Wen, Shona Osborne, Mark Zanin, Pei Low, Hai-Tao Wang, Simone Schoenwaelder, Shaun Jackson, Roland Wedlich-Soldner, Bart Vanhaesebroeck, Damien Keating, Frederic Meunier

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    58 Citations (Scopus)

    Abstract

    Neurosecretory vesicles undergo docking and priming before Ca2+ -dependent fusion with the plasma membrane. Although de novo synthesis of phosphatidylinositol(4,5)bisphosphate (PtdIns(4,5)P 2) is required for exocytosis, its precise contribution is still unclear. Here we show that inhibition of the p110î isoform of PI3-kinase by IC87114 promotes a transient increase in PtdIns(4,5)P 2, leading to a potentiation of exocytosis in chromaffin cells. We then exploit this pathway to examine the effect of a transient PtdIns(4,5)P 2 increase on neurosecretory vesicles behaviour, outside the context of a secretagogue stimulation. Our results demonstrate that a rise in PtdIns(4,5)P 2 is sufficient to promote the mobilization and recruitment of secretory vesicles to the plasma membrane via Cdc42-mediated actin reorganization. PtdIns(4,5)P 2, therefore, orchestrates the actin-based conveyance of secretory vesicles to the plasma membrane.

    Original languageEnglish
    Article number491
    Pages (from-to)491
    JournalNature Communications
    Volume2
    Issue number1
    DOIs
    Publication statusPublished - 2011

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