Photo-hydrogen evolution by immobilized marine green algae platymonas subcordiformis

Zhimin Zhang, Meifang Jin, Xingju Yu, Wei Zhang

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1 Citation (Scopus)


Platymonas subcordiformis was immobilized in the alginate-gel beads under the addition of 15 μmol·L-1 proton gradient uncoupler CCCP for H2 evolution. Among 5 different metal cations acted as gel bead stabilizing agents, calcium alginate beads increased H2 evolution activity by 20% compared with the control. The optimum bead diameter for photo-hydrogen evolution is 1 mm. At a wide range of cell loading the hydrogen yield is stable. The optimum pH range is enlarged with a shift of 0.5-1 pH to the acidic conditions. The optimum temperature and light intensity are 25°C and 4000 lx. After hydrogen evolution, the beads washed with 2% DMSO were re-cultured for 7 days and showed a similar photo-hydrogen evolution activity.

Original languageEnglish
Pages (from-to)80-84
Number of pages5
JournalHuagong Xuebao/Journal of Chemical Industry and Engineering (China)
Issue numberSUPPL.
Publication statusPublished - 1 Oct 2004
Externally publishedYes


  • CCCP (carbonyl cyanide m-chlorophenylhydrazone)
  • Hydrogen
  • Immobilized cells
  • Marine green microalgae
  • Platymonas subcordiformis


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