Platymonas subcordiformis was immobilized in the alginate-gel beads under the addition of 15 μmol·L-1 proton gradient uncoupler CCCP for H2 evolution. Among 5 different metal cations acted as gel bead stabilizing agents, calcium alginate beads increased H2 evolution activity by 20% compared with the control. The optimum bead diameter for photo-hydrogen evolution is 1 mm. At a wide range of cell loading the hydrogen yield is stable. The optimum pH range is enlarged with a shift of 0.5-1 pH to the acidic conditions. The optimum temperature and light intensity are 25°C and 4000 lx. After hydrogen evolution, the beads washed with 2% DMSO were re-cultured for 7 days and showed a similar photo-hydrogen evolution activity.
|Number of pages||5|
|Journal||Huagong Xuebao/Journal of Chemical Industry and Engineering (China)|
|Publication status||Published - 1 Oct 2004|
- CCCP (carbonyl cyanide m-chlorophenylhydrazone)
- Immobilized cells
- Marine green microalgae
- Platymonas subcordiformis