Photobiological hydrogen production by marine green alga Platymonas subcordiformis under carbonylcyanide-m-chlorophenylhydrazone

Chunqiu Ran, Xingju Yu, Meifang Jin, Wei Zhang

Research output: Contribution to journalArticle

Abstract

The marine green alga Platymonas subcordiformis can produce a significant amount of hydrogen under anaerobic condition and the addition of a proton gradient uncoupler, carbonylcyanide-m-chlorophenylhydrazone (CCCP). About 7.54 ml H2 was produced at 3×106 cells · ml-1 for 12 h in a 760 ml photobioreactor when 15 μmol·L-1 CCCP was added at the beginning of light illumination. During the stage of hydrogen evolution, the extracellular pH and the photochemical activity of PS II reduced from 7.62 to 7.49 and from 0.342 to 0.002, respectively. The system held at a very low oxygen level. These results indicate that the proton gradient uncoupler CCCP can facilitate the proton transfer across the thylakoid membrane, thus enhance the accessibility of H+ to hydrogenase, and keet anaerobic condition during hydrogen production through markedly inhibition of the PS II activity.

Original languageEnglish
Pages (from-to)108-112
Number of pages5
JournalHuagong Xuebao/Journal of Chemical Industry and Engineering (China)
Volume55
Issue numberSUPPL.
Publication statusPublished - 1 Oct 2004
Externally publishedYes

Keywords

  • Anaerobic
  • Carbonylcyanide-m-chlorophenylhydrazone
  • Hydrogen
  • Platymonas subcordiformis
  • PS II
  • Reversible hydrogenase

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